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Method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanut skin

A selenium-enriched protein, synchronous extraction technology, applied in chemical instruments and methods, peptide preparation methods, organic chemistry, etc., can solve the problem of single extraction of peanut coat and other problems, and achieve shortened extraction time, high extraction rate, and improved extraction rate. Effect

Active Publication Date: 2015-11-18
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to improve the utilization rate and economic value of the black peanut coat and solve the problem of the single extraction substance of the peanut coat, the present invention provides a method for synchronously extracting proanthocyanidin and selenium-enriched protein from the black peanut coat. The technical scheme is: liquid nitrogen freezing ultrafine pulverization of black peanut shells, microwave-assisted extraction of selenoproteins, and ultrasonic-assisted extraction of proanthocyanidins. The steps include:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Freeze 100g of black peanut shells with liquid nitrogen for 10 minutes and then carry out ultra-fine crushing; the crushed particle size should be below 10 μm. Add 200ml of 70% ethanol to the crushed peanut shells, and carry out microwave extraction in a microwave synthesis / catalysis instrument. The extraction conditions The microwave power is 350w, the microwave temperature is 40°C, and the microwave time is 6min. After extraction and filtration, the filtrate is concentrated in vacuum and then spray-dried. The spray-drying conditions are that the feed temperature is 40°C, the inlet air temperature is 160°C, and the air inlet volume is 25m 3 / h, black peanut clothing proanthocyanidin powder is obtained after drying. Add 200ml of alkali solution with pH 8 to the filter residue, stir evenly, and then ultrasonically treat at 60°C for 6 minutes, and adjust the pH of the supernatant to 4. Stand still for 10 minutes, centrifuge, precipitate through column chromatography G-25...

Embodiment 2

[0024] Freeze 100g of black peanut shells with liquid nitrogen for 10 minutes and then carry out ultra-fine crushing; the crushed particle size should be below 10 μm. Add 300ml of 60% ethanol to the crushed peanut shells, and carry out microwave extraction in a microwave synthesis / catalysis instrument. The extraction conditions The microwave power is 450w, the microwave temperature is 45°C, and the microwave time is 4min. After extraction and filtration, the filtrate is concentrated in vacuum and then spray-dried. The spray-drying conditions are that the feed temperature is 40°C, the inlet air temperature is 170°C, and the air inlet volume is 23m 3 / h, after the drying is completed, the black peanut clothing proanthocyanidin powder (1) is obtained. Add 300ml of alkaline solution with a pH of 8.5 to the filter residue, stir evenly, and then ultrasonically treat it at 50°C for 12 minutes, and adjust the pH of the supernatant to 4.5. Stand still for 10 minutes, centrifuge, preci...

Embodiment 3

[0029] Freeze 100g of black peanut shells with liquid nitrogen for 10 minutes and then carry out ultra-micro-broken crushing; the crushed particle size should be below 10 μm. Add 400ml of 70% ethanol to the crushed peanut shells, and perform microwave extraction in a microwave synthesis / catalysis instrument. The extraction conditions The microwave power is 550w, the microwave temperature is 50°C, and the microwave time is 2min. After extraction and filtration, the filtrate is concentrated in vacuum and then spray-dried. The spray-drying conditions are that the feed temperature is 40°C, the inlet air temperature is 180°C, and the air inlet volume is 25m 3 / h, black peanut clothing proanthocyanidin powder is obtained after drying. Add 400ml of alkali solution with pH 9 to the filter residue, stir evenly, and then ultrasonically treat at 40°C for 12 minutes, and adjust the pH of the supernatant to 5. Stand still for 10 minutes, centrifuge, precipitate through column chromatograp...

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Abstract

The invention discloses a method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanut skin. Firstly, the black peanut skin subjected to liquid nitrogen freezing superfine grinding, then ultrasonic-assisted extraction of the proanthocyanidin and ultrasonic-assisted extraction of the selenium-enriched protein are performed simultaneously, prepared proanthocyanidin powder is high in proanthocyanidin content and high in activity, and the selenium-enriched protein is high in organic selenium content and high in activity. With the adoption of the method for preparing two active substances (the proanthocyanidin and the selenium-enriched protein) from the raw material, namely, the black peanut skin, the technological process can be simplified, the raw material cost can be reduced, the working efficiency can be improved, in addition, the method is high in extraction rate and suitable for industrial production, and the activity of extracted substances is high.

Description

technical field [0001] The invention relates to a method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanuts, and belongs to the field of reuse of peanut processing by-products. Background technique [0002] Black peanuts are peanuts whose underwear is black. The underwear is black and translucent. Other parts such as peanut shells and peanut kernels are no different from ordinary peanuts. Black peanuts are reported to be rich in nutrients. Compared with ordinary peanuts, they contain more amino acids, are rich in selenium, have high protein content, high yield, and strong disease resistance. Therefore, they are well received by growers and users. The planting area increase yearly. According to relevant literature reports, black peanuts have the functions of strengthening the kidney and strengthening the body, removing excess lipids in the body, anti-oxidation, and delaying aging. Its main functional active substances exist in the ...

Claims

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Application Information

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IPC IPC(8): C07D311/62C07K1/36C07K1/30C07K1/16
CPCC07D311/62C07K1/16C07K1/30C07K1/36
Inventor 张初署孙杰于丽娜毕洁
Owner SHANDONG PEANUT RES INST
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