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Methylene blue thermosensitive hydrogel and its preparation and application in anatomic hepatectomy

A temperature-sensitive hydrogel and methylene blue technology, applied in the field of dyeing agents, can solve problems such as uneven dyeing, affecting surgical operations, and shallow staining of liver tissue, achieving good dyeing effects and increasing the specific surface area

Active Publication Date: 2018-11-20
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in clinical practice, it has been found that methylene blue metabolizes quickly, the staining of liver tissue is lighter, the staining is uneven and fades quickly, and when it enters the liver parenchyma, it is often impossible to judge the boundary
Therefore, multiple injections are often required in the operation, and the injection process often overflows to the local operation, and the aspirator is not easy to suck clean, which seriously affects the operation.

Method used

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  • Methylene blue thermosensitive hydrogel and its preparation and application in anatomic hepatectomy
  • Methylene blue thermosensitive hydrogel and its preparation and application in anatomic hepatectomy

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preparation example Construction

[0028] 1) Preparation of copolymers: Weigh a certain amount of DL-LA, GA and PEG1500 and place them in an oven at 35-40°C for 1.5 hours, and then dry them at 120-140°C. Dry in the oven of ℃ for 0.5h, mix and pack in the dry 50mL vial after drying finishes, and add catalyzer stannous isooctanoate, the consumption of described catalyzer stannous isooctanoate is 0.2wt% of raw material gross mass, then put Place the vials into a freeze dryer for drying. The freeze drying temperature is -10 to -30°C, and the drying time is 1.5 to 2.0 hours. After vacuuming, the cap is taken out, and the cap is crimped and sealed with a penicillin bottle electric capping machine. React at 120-135°C for 13-14 hours, dissolve the obtained product in pure water at 5°C, heat to 75-80°C to precipitate the copolymer, discard the water layer, and repeat the operation 3 times to remove unreacted monomers and low molecular weight The water-soluble substance of the obtained copolymer is freeze-dried for 25-28...

Embodiment 1

[0041] A methylene blue temperature-sensitive hydrogel, wherein the hydrogel is a temperature-sensitive polyglycolide-lactide-polyethylene glycol-polyglycolide-lactide gel loaded with methylene blue, and the methylene blue The weight-number ratio of the blue to the temperature-sensitive polyglycolide lactide-polyethylene glycol-polyglycolide lactide gel is 18:82.

[0042] The preparation method of the methylene blue thermosensitive hydrogel comprises steps:

[0043] 1) Preparation of copolymer: Weigh a certain amount of DL-LA, GA, and PEG1500 in an oven at 38°C for 1.5 hours, and then place them in an oven at 130°C Dry for 0.5h, mix and pack in the dry 50mL cillin bottle after drying, and add catalyst stannous isooctanoate, the consumption of described catalyzer stannous isooctanoate is 0.2wt% of raw material gross mass, then put the cillin bottle into Freeze drying machine for drying, freeze-drying temperature is -20°C, drying time is 2.0h, after being vacuumed, the cap is t...

Embodiment 2

[0048] A methylene blue temperature-sensitive hydrogel, wherein the hydrogel is a temperature-sensitive polyglycolide-lactide-polyethylene glycol-polyglycolide-lactide gel loaded with methylene blue, and the methylene blue The weight-number ratio of the blue to the temperature-sensitive polyglycolide lactide-polyethylene glycol-polyglycolide lactide gel is 15:85.

[0049] The preparation method of the methylene blue thermosensitive hydrogel comprises steps:

[0050] 1) Preparation of copolymer: Weigh a certain amount of DL-LA, GA, and PEG1500 in an oven at 40°C for 1.5 hours, and then place them in an oven at 140°C Dry for 0.5h, mix and pack in the dry 50mL cillin bottle after drying, and add catalyst stannous isooctanoate, the consumption of described catalyzer stannous isooctanoate is 0.2wt% of raw material gross mass, then put the cillin bottle into Freeze dryer for drying, freeze-drying temperature is -30°C, drying time is 1.5h, take out the cap after evacuation, use a pe...

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Abstract

The invention discloses methylene blue temperature-sensitive hydrogel, preparation thereof, and an application thereof in anatomical hepatectomies. The methylene blue temperature-sensitive hydrogel is temperature-sensitive poly(glycolide-co-lactide)-polyethylene glycol-poly(glycolide-co-lactide) gel loaded with methylene blue. A preparation method of the methylene blue temperature-sensitive hydrogel comprises the following steps: synthesis of temperature-sensitive poly(glycolide-co-lactide)-polyethylene glycol-poly(glycolide-co-lactide) copolymer, preparation of blank gel, pretreatment of methylene blue, and preparation of the methylene blue temperature-sensitive hydrogel. The methylene blue temperature-sensitive hydrogel can be injected into a to-be-cut hepatic tissue through portal vein injection, and gradually forms a gelatinization state after reaching the in-vivo body, and achieves the slow release and positioning effects at the diseased region, and the to-be-cut hepatic tissue is dyed permanently, so that purpose of the accurate anatomical hepatectomies is achieved.

Description

technical field [0001] The present invention relates to a dye used in precise anatomical hepatectomy. More specifically, the present invention relates to a methylene blue thermosensitive hydrogel used in precise anatomical hepatectomy and its preparation method. Background technique [0002] Accurate anatomical hepatectomy is the most ideal surgical method for liver cancer, and the precise definition of the interface of hepatectomy is the core of anatomical hepatectomy. Accurate anatomical hepatectomy can help reduce intraoperative bleeding due to liver parenchymal section along the liver segment or the gap between liver lobes; at the same time, because liver cancer has a biological tendency to spread along the portal vein branch of the tumor-bearing liver segment It is easy to invade the paracancerous portal vein, forming hidden portal vein tumor thrombus and forming satellite lesions in the area dominated by the portal vein. Anatomical hepatic (segmental) resection is in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K31/5415A61K47/34A61P41/00
Inventor 许月芳朱自满刘皈阳李翔
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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