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Construction method of systemic lupus erythematosus map model

A lupus erythematosus, construction method technology, applied in biochemical equipment and methods, library creation, determination/inspection of microorganisms, etc., can solve problems such as difficult to detect

Active Publication Date: 2015-11-11
中国人民解放军联勤保障部队第九二四医院
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Problems solved by technology

And even if there is a difference, it is difficult to detect in the peripheral blood

Method used

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  • Construction method of systemic lupus erythematosus map model
  • Construction method of systemic lupus erythematosus map model
  • Construction method of systemic lupus erythematosus map model

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Embodiment Construction

[0048] In order to make the above objects, features and advantages of the present invention more comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described here, and those skilled in the art can make similar improvements without departing from the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0049] For example, a method for constructing a systemic lupus erythematosus map model includes the following steps: setting a systemic lupus erythematosus group and a normal control group, each group including several peripheral blood samples from different human bodies that have been sep...

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Abstract

The invention relates to a construction method of a systemic lupus erythematosus map model. The construction method comprises: setting a systemic lupus erythematosus group and a control group, wherein each group comprises a plurality of peripheral blood samples isolated from human body and derived from different people; respectively extracting DNA from each peripheral blood sample to obtain DNA liquids to be detected; determining the DNA contents and the DNA integrity in the DNA liquids to be detected, detecting whether the sample is qualified, and performing the next step if the sample is qualified; constructing a gene library by using a multiplex PCR technology, detecting whether the gene library is qualified, and performing the next step if the gene library is qualified; carrying out sequencing on the gene library by using a high-throughput sequencing technology; and analyzing the sequencing results, and constructing the systemic lupus erythematosus map model. According to the construction method of the systemic lupus erythematosus map model of the present invention, the design is reasonable and feasible, and the SLE related information adopted as the middle results can be effectively obtained.

Description

technical field [0001] The invention relates to an acquisition of systemic lupus erythematosus information as an intermediate result, in particular to a method for constructing a systemic lupus erythematosus map model. Background technique [0002] Systemic lupus erythematosus (systemic lupus erythematosus, SLE) is a classic autoimmune disease, characterized by autoantibody production, complement activation, immune complex deposition and various tissue and organ damage. The clinical manifestations of SLE are diverse, and multiple tissues and organs such as the skin, kidney, hematopoietic system, nervous system, lung, and serosa can be involved. There are many kinds of abnormal immune functions in patients, such as decreased number and function of T cells; excessive activation and proliferation of B cells, production of various autoantibodies; dysfunction of immune complex clearance, deficiency of complement system; abnormal apoptosis and production of a large number of cytok...

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Application Information

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IPC IPC(8): C40B50/06C12Q1/68
Inventor 眭维国戴勇侯显良
Owner 中国人民解放军联勤保障部队第九二四医院
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