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Herpes simplex virus keratitis vaccine and preparation method thereof

A technology for viral keratitis and herpes simplex, applied in antiviral agents, sensory diseases, pharmaceutical formulations, etc., can solve problems such as differences in immune effects, achieve great social benefits, have good solution plasticity, and enhance antigen presentation efficiency Effect

Active Publication Date: 2018-01-16
SOUTHEAST UNIV
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Problems solved by technology

[0005] The immune effect caused by different vaccination routes is also different

Method used

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  • Herpes simplex virus keratitis vaccine and preparation method thereof
  • Herpes simplex virus keratitis vaccine and preparation method thereof

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Embodiment Construction

[0052] The present invention will be further described below in combination with specific embodiments.

[0053] The herpes simplex virus keratitis vaccine provided by the present invention is composed of plasmid pRSC-NLDC145*gD-IL-21, which is wrapped with nanomaterial chitosan; wherein: gD is one of the main envelope glycoproteins of herpes simplex virus type I , using the genome of the extracellular region; NLDC-145 is a monoclonal antibody against mouse DEC-205, which is a small molecular weight, strong penetrating power, can avoid non-specific cytotoxicity and immunogenicity, and is modified by DC in vivo Ideal carrier; IL-21 is an immune adjuvant.

[0054] Among them, the concentration of plasmid pRSC-NLDC145*gD-IL-21 was 5 μg / μl.

[0055] What the present invention provides is to construct a DNA vaccine targeting DC, which fuses the DEC-205 receptor monoclonal antibody NLDC-145 on the surface of DC with the main immunogen gD of HSV-1, and retains the previous recombinan...

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Abstract

The invention discloses a herpes simplex keratitis (HSK) vaccine and a preparation method thereof. The vaccine is composed of plasma pRSC-NLDC145*gD-IL-21 and is wrapped by nanomaterial chitosan; wherein, gD is one of the main envelope glycoproteins of herpes simplex virus type I and adopts extracellular region genome; NLDC-145 is a monoclonal antibody aiming at mouse DEC-205; the IL-21 is an immunologic adjuvant. The vaccine is prepared by adopting a series of steps, namely obtainment of HSV-1gD and NLDC145 genes; establishment of pRSC-NLDC145*gD-IL-21 plasma; preparation of a chitosan / plasma DNA nano-vaccine, and the like. The HSK vaccine disclosed by the invention can be used for resisting inflection of HSV-1 safely and effectively and remarkably restricting the occurrence and development of HSK and has a broad application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a herpes simplex virus keratitis vaccine and a preparation method thereof. Background technique [0002] Herpes simplex keratitis (HSK) is a keratitis mainly caused by HSV-1 infection of the eye. One of the most common blinding eye diseases. According to the survey, in my country, corneal blindness ranks second only to cataract blindness, while HSK blindness ranks first, accounting for about 42.85%; in developed countries, the incidence rate is 5.9 / 10 per year. 5 ~20.7 / 10 5 , with a prevalence rate of 149 / 10 5 . HSV-1 often causes blepharitis and keratoconjunctivitis after the initial infection, and the virus tends to lurk in local ganglia, mainly the trigeminal ganglion (TG). When steroid hormones and immunosuppressants are used, the virus will be reactivated, resulting in recurrent HSK. According to statistics by some scholars, the 3-year recurrence rate can reach 33.5%. In rece...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/245A61K48/00A61K39/395A61K39/39A61K47/36C12N15/66C12N15/70A61P31/22A61P27/02
Inventor 胡凯
Owner SOUTHEAST UNIV
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