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Herpes simplex keratitis (HSK) vaccine and preparation method thereof

A technology for viral keratitis and herpes simplex virus, which is applied in the field of herpes simplex virus keratitis vaccine and its preparation, can solve the problems of differences in immune effects, achieve great social benefits, enhance antigen presentation efficiency, and strong specificity The effect of the immune effect

Active Publication Date: 2015-11-11
SOUTHEAST UNIV
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  • Claims
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Problems solved by technology

[0005] The immune effect caused by different vaccination routes is also different

Method used

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  • Herpes simplex keratitis (HSK) vaccine and preparation method thereof
  • Herpes simplex keratitis (HSK) vaccine and preparation method thereof

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Embodiment Construction

[0052] The present invention will be further described below in conjunction with specific embodiments.

[0053] The herpes simplex virus keratitis vaccine provided by the invention is composed of plasmid pRSC-NLDC145*gD-IL-21, and is wrapped with nanomaterial chitosan; wherein: gD is one of the main envelope glycoproteins of herpes simplex virus type I , using the extracellular region of the genome; NLDC-145 is a monoclonal antibody against mouse DEC-205, it is a small molecular weight, strong penetrating power, can avoid non-specific cytotoxicity and immunogenicity, is modified by DC in vivo Ideal carrier; IL-21 is an immune adjuvant.

[0054] The concentration of plasmid pRSC-NLDC145*gD-IL-21 was 5 μg / μl.

[0055] The present invention provides a DNA vaccine targeting DC, fuses the DEC-205 receptor monoclonal antibody NLDC-145 on the DC surface with gD, the main immunogen of HSV-1, and retains the previous recombinant plasmid PRSC-gD-IL- The immune-enhancing molecule and a...

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Abstract

The invention discloses a herpes simplex keratitis (HSK) vaccine and a preparation method thereof. The vaccine is composed of plasma pRSC-NLDC145*gD-IL-21 and is wrapped by nanomaterial chitosan; wherein, gD is one of the main envelope glycoproteins of herpes simplex virus type I and adopts extracellular region genome; NLDC-145 is a monoclonal antibody aiming at mouse DEC-205; the IL-21 is an immunologic adjuvant. The vaccine is prepared by adopting a series of steps, namely obtainment of HSV-1gD and NLDC145 genes; establishment of pRSC-NLDC145*gD-IL-21 plasma; preparation of a chitosan / plasma DNA nano-vaccine, and the like. The HSK vaccine disclosed by the invention can be used for resisting inflection of HSV-1 safely and effectively and remarkably restricting the occurrence and development of HSK and has a broad application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a herpes simplex virus keratitis vaccine and a preparation method thereof. Background technique [0002] Herpes simplex keratitis (HSK) is a kind of keratitis mainly caused by HSV-1 infection of the eye, which occupies the first place in corneal diseases. one of the blinding eye diseases. According to the survey, in my country, corneal blindness ranks second after cataract blindness, and HSK blindness ranks first, accounting for about 42.85%; the incidence rate in developed countries is 5.9 / 10 per year. 5 ~20.7 / 10 5 , the prevalence was 149 / 10 5 . HSV-1 often causes blepharitis and keratoconjunctivitis after the initial infection, and the virus is prone to latent in local ganglia, mainly the trigeminal ganglion (TG). Steroid hormones and immunosuppressive agents can cause the virus to be reactivated, resulting in recurrent HSK. According to some scholars statistics, the 3-year recu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61K48/00A61K39/395A61K39/39A61K47/36C12N15/66C12N15/70A61P31/22A61P27/02
Inventor 胡凯
Owner SOUTHEAST UNIV
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