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Application of seabuckthorn flavonoids in the preparation of compositions for enhancing the efficacy of opioids

A seabuckthorn flavonoid and composition technology, applied in the field of new drug use, can solve the problems of poor analgesic effect, strong adverse reactions, low dose, etc., and achieve the advantages of enhancing analgesic effect, prolonging analgesic time, and delaying opioid tolerance phenomenon. Effect

Active Publication Date: 2019-06-14
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Therefore, in the prior art, opioids have the obvious defects of strong adverse reactions at high doses and poor analgesic effect at low doses. In addition, opioids are widely tolerated

Method used

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  • Application of seabuckthorn flavonoids in the preparation of compositions for enhancing the efficacy of opioids
  • Application of seabuckthorn flavonoids in the preparation of compositions for enhancing the efficacy of opioids
  • Application of seabuckthorn flavonoids in the preparation of compositions for enhancing the efficacy of opioids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Systemic administration of sea buckthorn flavone can enhance the acute analgesic effect of systemic administration of morphine

[0035] a) Experimental animals: healthy adult female ICR mice, clean grade, weighing 18-22 g. The experimental animals were kept in an independent environment with alternating day and night for 12h-12h, the room temperature was maintained at 24±2℃, and they were free to drink and eat. The experiment was carried out after adapting to the environment for 1 week. All handling of animals followed the requirements of the Ethics Committee of the International Association for Pain Research.

[0036] b) Test drugs and reagents:

[0037] The drug seabuckthorn flavone, with a purity of 98%, is commercially available. Morphine hydrochloride was purchased from Shenyang No. 1 Pharmaceutical Factory.

[0038] c) Test method:

[0039] Dosage and method of sea buckthorn flavone and morphine administration: sea buckthorn flavone is dissolved in normal salin...

Embodiment 2

[0044] Example 2 Seabuckthorn flavonoids can alleviate opioid tolerance caused by chronic administration of morphine

[0045] a) Experimental animals:

[0046] Female ICR mice, clean grade, weighing 18-22g. The experimental animals were kept in an independent environment with alternating day and night for 12h-12h. The room temperature was maintained at 24±2℃, and they were free to drink and eat. The experiment was carried out after adapting to the environment for 1 week. All handling of animals followed the requirements of the Ethics Committee of the International Association for Pain Research.

[0047] b) Test drugs and reagents:

[0048] The drug seabuckthorn flavone, with a purity of 98%, is commercially available. Morphine hydrochloride was purchased from Shenyang No. 1 Pharmaceutical Factory.

[0049] c) Test method:

[0050] Dosage and method of sea buckthorn flavone administration: sea buckthorn flavone is dissolved in physiological saline, the dose is 60 mg / kg, and the adminis...

Embodiment 3

[0055] Example 3 Seabuckthorn flavone can inhibit morphine-induced up-regulation of multiple inflammatory factors in microglia

[0056] a) Test cells:

[0057] BV2 microglial cell culture in DMEM medium containing 10% fetal bovine serum, 5% CO 2 , 37 ℃ constant temperature incubator culture. It can be passaged when it covers 80% of the bottom surface of the culture flask. 24 hours before the experiment, the medium was changed to 0.5% fetal bovine serum high glucose DMEM.

[0058] b) Test drugs and reagents:

[0059] Fetal bovine serum was purchased from Hyclone; DMEM and Trizol were purchased from Invitrogen; TaKaRa PrimeScript kit was purchased from Dalian Bao Biological Engineering Co., Ltd.

[0060] c) Test method:

[0061] Real-time quantitative PCR: Trizol method was used to extract RNA from each group of cells. The reverse transcription reaction uses the SYBR Green Assay of the TaKaRaPrimeScript kit. The reaction system is: 5×PrimeScript Buffer 2μl, Prime Script RT Enzyme 0.5μl,...

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Abstract

The invention discloses an application of seabuckthorn flavone in the preparation of compositions for enhancing the drug effect of opioids. Compared with applications in the prior art, the application has the advantages of substantial prolongation of the pain easing time of the opioids, enhancement of the pain easing effect of the opioids, and delaying of opioid tolerance caused by the opioids through synergism realized by common use of the seabuckthorn flavone and the opioids.

Description

Technical field [0001] The invention relates to the application of seabuckthorn flavonoids in the preparation of a composition for enhancing the efficacy of opioids, and belongs to the field of new uses of medicines. Background technique [0002] Pain is a complex physiological and psychological activity, including the pain sensation caused by noxious stimuli acting on the body, and the body's pain response to noxious stimuli. Pain can be used as a warning of injury to the body, causing a series of defensive protective reactions, but certain acute or long-term severe pain is an unbearable torture to the body. Pain is one of the most common symptoms in clinic, and it is also an important problem that medical workers urgently need to solve. [0003] In terms of the course of the disease, pain can be divided into acute pain and chronic pain. Acute pain is pain that has recently occurred and has a short duration, usually related to injury or disease. Chronic pain is pain that lasts ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61K45/00A61K31/485A61P25/04
Inventor 季浩刘文涛阚建伟窦长清
Owner JIANGSU TIANSHENG PHARMA
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