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Application of seabuckthorn flavone in preparation of compositions for enhancing drug effect of opioids

A technology of opioids and seabuckthorn flavonoids, applied in drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve the problems of strong adverse reactions, poor analgesic effect, and low dosage, so as to prolong the analgesic time and delay opioid tolerance Phenomenon, enhanced analgesic effect

Active Publication Date: 2015-11-11
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Therefore, in the prior art, opioids have the obvious defects of strong adverse reactions at high doses and poor analgesic effect at low doses. In addition, opioids are widely tolerated

Method used

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  • Application of seabuckthorn flavone in preparation of compositions for enhancing drug effect of opioids
  • Application of seabuckthorn flavone in preparation of compositions for enhancing drug effect of opioids
  • Application of seabuckthorn flavone in preparation of compositions for enhancing drug effect of opioids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Systemic administration of seabuckthorn flavonoids can enhance the acute analgesic effect of systemic administration of morphine

[0035] a) Experimental animals: healthy adult female ICR mice, clean grade, weighing 18-22 g. The experimental animals were raised in an independent environment with 12h-12h alternation of day and night, the room temperature was maintained at 24±2°C, water and food were free to drink, and the experiment was carried out after 1 week of adaptation to the environment. All handling of animals followed the requirements of the Ethics Committee of the International Association for the Study of Pain.

[0036] b) Test drugs and reagents:

[0037] Drug Seabuckthorn flavonoids, with a purity of 98%, are commercially available. Morphine hydrochloride was purchased from Shenyang No. 1 Pharmaceutical Factory.

[0038] c) Test method:

[0039] Dosage and method of administration of seabuckthorn flavonoids and morphine: seabuckthorn flavonoids...

Embodiment 2

[0044] Example 2 Seabuckthorn flavonoids can alleviate opioid tolerance caused by chronic administration of morphine

[0045] a) Test animals:

[0046] Female ICR mice, clean grade, weighing 18-22 g. The experimental animals were raised in an independent environment with 12h to 12h alternation of day and night, the room temperature was maintained at 24±2°C, and they had free access to water and food. Experiments were carried out after 1 week of adaptation to the environment. All handling of animals followed the requirements of the Ethics Committee of the International Association for the Study of Pain.

[0047] b) Test drugs and reagents:

[0048] Drug Seabuckthorn flavonoids, with a purity of 98%, are commercially available. Morphine hydrochloride was purchased from Shenyang No. 1 Pharmaceutical Factory.

[0049] c) Test method:

[0050] Dosage and method of administration of seabuckthorn flavonoids: seabuckthorn flavonoids are dissolved in physiological saline, the dose...

Embodiment 3

[0055] Example 3 Seabuckthorn flavonoids can inhibit the up-regulation of various inflammatory factor mRNAs in microglial cells induced by morphine

[0056] a) Test cells:

[0057] BV2 microglial cells were cultured in DMEM medium containing 10% fetal bovine serum, 5% CO 2 , 37 ℃ constant temperature incubator culture. It can be subcultured when it covers 80% of the bottom of the culture bottle. 24 hours before the experiment, the medium was replaced with 0.5% fetal bovine serum high glucose DMEM.

[0058] b) Test drugs and reagents:

[0059] Fetal bovine serum was purchased from Hyclone; DMEM and Trizol were purchased from Invitrogen; TaKaRaPrimeScript kit was purchased from Dalian Bao Biological Engineering Co., Ltd.

[0060] c) Test method:

[0061] Real-time quantitative PCR: the RNA of each group was extracted by Trizol method. The reverse transcription reaction uses SYBRGreenAssay of TaKaRaPrimeScript kit, the reaction system is: 5×PrimeScriptBuffer2μl, PrimeScript...

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Abstract

The invention discloses an application of seabuckthorn flavone in the preparation of compositions for enhancing the drug effect of opioids. Compared with applications in the prior art, the application has the advantages of substantial prolongation of the pain easing time of the opioids, enhancement of the pain easing effect of the opioids, and delaying of opioid tolerance caused by the opioids through synergism realized by common use of the seabuckthorn flavone and the opioids.

Description

technical field [0001] The invention relates to the application of seabuckthorn flavonoids in the preparation of compositions for enhancing the efficacy of opioids, and belongs to the field of new applications of medicines. Background technique [0002] Pain is a complex physiological and psychological activity, including the pain sensation caused by noxious stimuli acting on the body, and the body's pain response to noxious stimuli. Pain can be used as a warning that the body is injured, causing a series of defensive protective reactions of the body, but some acute or long-term severe pain is an unbearable torture to the body. Pain is one of the most common clinical symptoms, and it is also an important problem that medical workers need to solve urgently. [0003] In terms of the course of the disease, pain can be divided into acute pain and chronic pain. Acute pain is pain that is new and of short duration, usually associated with injury or disease. Chronic pain refers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K45/00A61K31/485A61P25/04
Inventor 季浩刘文涛阚建伟窦长清
Owner JIANGSU TIANSHENG PHARMA
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