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A kind of preparation method of binary sucrase inhibitor

A sucrase and inhibitor technology, applied in the field of preparation of binary sucrase inhibitors, can solve the problems of high proportion of functional healthy sugar arabinose, complex operation steps, low product, etc., to improve inhibition efficiency and strong technical operability , cost reduction effect

Active Publication Date: 2018-01-19
广西壮族自治区蚕业技术推广总站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the method disclosed in Chinese patent CN1579445A, 1-deoxynojirimycin with a content of 1-3% is obtained mainly by adding a flocculant and then undergoing alcohol precipitation. The main disadvantage of this method is that all the target products are relatively low
Chinese patent CN1430964A discloses the extraction method of 1-deoxynojirimycin, which is mainly extracted and concentrated in the pretreatment process until the specific gravity of the extract is 1.12-1.2, adding 1 to 5 times the amount of the original drug to dissolve in water, and then adding flocculant after adjusting the pH Stand still for 12 hours, then go through ion exchange resin deionization, stand for 4 hours and reflux extraction; this technology has the disadvantages of complicated operation steps and low concentration of the obtained product
[0004] Chinese patent CN101897420A discloses a preparation method of a new functional healthy sugar, which is to mix L-arabinose or arabinose solution with sucrose or sucrose solution at a ratio of 2-98:98-2 to obtain 20-100 The target product, the proportion of some new functional healthy sugar arabinose prepared by this method is too high, resulting in high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An example of the preparation method of binary invertase inhibitor of the present invention, comprises the steps:

[0027] Preparation of mulberry leaf sucrase inhibitor:

[0028] Smash strong mulberry leaves to obtain broken mulberry leaves, add 3 times (W / W) purified water to grind to obtain mulberry leaf pulp, add 0.1% cellulase, 0.05% pectinase at a temperature of 50 ° C, enzymolysis for 3 hours, and then At centrifugal radius r=φ1500mm, motor speed=3000r / min, centrifugal separation obtains separation liquid, separation liquid is filtered with carbon rod filter to obtain filtrate, filtrate is added in the weakly basic ion exchange fiber with speed=5ml / s, then with The sodium carbonate aqueous solution of pH=8.0 is desorbing agent, and desorption speed=45ml / s, and desorbing agent consumption is 2 times of volume on the ion-exchange fiber that adsorbs 1-deoxynojirimycin, and the desorbing solution is concentrated to paste and promptly obtains containing 1-deoxynojirim...

Embodiment 2

[0032] Another example of the preparation method of the binary sucrase inhibitor of the present invention comprises the following steps:

[0033] Preparation of mulberry leaf sucrase inhibitor:

[0034] Smash robust mulberry leaves to obtain crushed mulberry leaves, add 8 times (W / W) purified water to grind to obtain mulberry leaf pulp, add 0.2% cellulase, 0.08% pectinase at a temperature of 60 ° C, enzymolysis for 5 hours, and then At the centrifugal radius r=φ800mm, motor speed=2950r / min, centrifugation to obtain the separation liquid, the separation liquid is filtered with a carbon rod filter to obtain the filtrate, and the filtrate is added to the weakly basic ion exchange fiber with speed=15ml / s, and then The sodium carbonate aqueous solution of pH=8.5 is desorbent, and desorption speed=75ml / s, and desorbent consumption is 3 times of volumes on the ion exchange fiber that adsorbs 1-deoxynojirimycin, and desorbing solution is concentrated to paste and promptly obtains cont...

Embodiment 3

[0038] Another example of the preparation method of the binary sucrase inhibitor of the present invention comprises the following steps:

[0039] Preparation of mulberry leaf sucrase inhibitor:

[0040] Smash robust mulberry leaves to obtain crushed mulberry leaves, add 6 times (W / W) purified water to grind to obtain mulberry leaf pulp, add 0.15% cellulase, 0.06% pectinase at a temperature of 55 ° C, and enzymolyze for 4 hours, Then at centrifugal radius r=φ1000mm, motor speed=2950r / min, centrifugation obtains separated liquid, separated liquid is filtered with carbon rod filter to obtain filtrate, filtrate is added in the weakly basic ion exchange fiber with speed=10ml / s, then Use sodium carbonate aqueous solution with pH = 8.2 as desorbent, desorption rate = 60ml / s, and use 2.5 times the volume on ion exchange fibers adsorbing 1-deoxynojirimycin. The desorption solution is concentrated to a paste containing 1-deoxynojirimycin Mymycin 29.7% Mulberry Leaf Concentrate.

[004...

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Abstract

The invention relates to the field of food and biotechnology, in particular to a preparation method of a binary sucrase inhibitor, comprising the following steps: crushing fresh mulberry leaves, refining, enzymatic hydrolysis, separation, ion exchange fiber extraction, and concentration to obtain 25-30% 1-deoxynojirimycin mulberry leaf concentrate, then mix the mulberry leaf concentrate and L-arabinose crystals at a ratio of 1:4500-5000, melt, stir evenly and place in a vacuum desiccator Dried for 2-4 hours, crushed and sieved to obtain 100-150 meshes of binary invertase inhibitor, the binary invertase inhibitor has water instant solubility, high rate of inhibiting invertase, and can better inhibit the hydrolysis of sucrose in the stomach. Wide application prospects.

Description

technical field [0001] The invention relates to a preparation method of a binary invertase inhibitor, in particular to a method for preparing the binary invertase inhibitor by compounding 1-deoxynojirimycin and L-arabinose from mulberry leaves. Background technique [0002] Mulberry leaf is the leaf of Moraceae (Moraceae) Morus plant (Morus L.). It is a dual-use resource for medicine and food announced by the Ministry of Health. It is cultivated and produced in various regions in southern my country, such as Guangdong, Guangxi, and Zhejiang. Mulberry leaves are rich in resources in my country and are a commonly used traditional Chinese medicine in ancient medical prescriptions. Mulberry leaves are bitter in taste, sweet, cold in nature, and return to the lung and liver meridian. Based on the study of the chemical components of mulberry leaves and animal experiments, it has been found that mulberry leaves have the functions of lowering blood pressure, lowering blood sugar, l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/605A61K31/445A61P3/10A23L33/00
Inventor 梁贵秋李全祁广军陆飞陆春霞董桂清吴婧婧周晓玲林刚黄正勇
Owner 广西壮族自治区蚕业技术推广总站
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