Fluorophore Derivatives Used for Determination of Polysulfurized Cysteine Fluoropyrrole Fluorophore and Its Application
A cysteine fluoroboropyrrole, cysteine technology, applied in the field of fluorescent probes, can solve problems such as the lack of development of fluorescent probes, reduce sample processing time, reduce detection costs, and improve detection accuracy. Effect
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Embodiment 1
[0040] Example 1. Preparation of polysulfide cysteine fluoroboron pyrrole fluorophore (BODIPY) derivatives:
specific Embodiment
[0041] The fluorophore (BODIPY) derivatives used for the determination of polysulfide cysteine shown in the general formula Ⅰ are reacted with commercially available p-hydroxyacetophenone and benzaldehyde to synthesize the corresponding fluorophore BODIPY, and then in the fluorescence The corresponding positions of the groups are respectively modified with different positioning groups. Finally, the fluorophore of the modified positioning group is reacted with dithiophthalic acid in dichloromethane solvent, catalyzed by DMAP and EDC, and then reduced by dithiothreitol to generate the corresponding polysulfide cysteine. Fluoropyrrole-like fluorophore (BODIPY) derivatives. Specific examples are as follows:
[0042] Preparation of a compound of formula:
[0043] Under the protection of argon, the BODIPY fluorophore (92.6mg, 0.1mmol) with triphenylphosphine group, 2,2'-diselenodibenzoic acid (40.0mg, 0.1mmol), 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, ...
Embodiment 2
[0051] The prepared compound of formula one is used as a probe in water system, simulated physiological environment and intracellular for CysS n The detection of SSH simulates physiological conditions. The following experiments are all carried out under the condition of pH=7.4 (HEPES buffer solution, the concentration is 10 mM), and the probe concentration is 10 μM.
[0052] Above-mentioned preparation gained formula two compound pair CysS n Spectral response of SSH; add 10μM compound of formula 1 to 10ml colorimetric tube, then add 10mM HEPES, then add 0-10μM CysS n SSH, dilute to 10ml with ultrapure water, shake the solution to balance for 10min, then add the above working solution into the fluorescence dish to measure the fluorescence spectrum. Fluorescence spectral changes and UV spectra such as figure 1 shown. Depend on figure 1 The compound shown can be used to realize CysS in vivo n SSH detection. Simultaneously, the probe provided by the embodiment of the present...
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