Method for producing bound trotters through artificial inoculation and fermentation
A technology of artificial inoculation and hoof binding, which is applied in the field of food processing, can solve the problems that no fermented hoofs are sold, the taste of meat products is sour, and it is unacceptable, so as to inhibit the growth of pathogenic bacteria in the intestine, make the meat fresh and tender, and promote digestion absorption effect
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[0030] Preparation of starter:
[0031] Lactobacillus bulgaricus was cultured statically at 32-37°C for 20-24 hours until logarithmic growth phase using MRS medium, Saccharomyces cerevisiae was cultured in YPD medium 28-32°C, 150r / min shaking for 14-20 hours, Staphylococcus carnosus was cultured with MSA Culture medium at 32-37°C, static culture for 20-24h to the logarithmic growth phase, after high-density enrichment of the three kinds of bacteria in the liquid medium, centrifuge at 4000r / min for 30min at 4°C to collect the sludge, and sterilize the physiological saline Wash 2 to 3 times and make 10 7 ~10 8 cfu / mL starter, set aside.
[0032] The formula of each culture medium is as follows:
[0033] MRS medium: peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, glucose 20g, sodium acetate 5g, diamine citrate 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000mL , pH6.2~6.4;
[0034] MSA medium: beef ex...
Embodiment 1
[0037] A method for artificially inoculating and fermenting to produce bound hooves, comprising the following steps:
[0038] A. Pig’s trotter skin preparation: select fresh pig’s trotters, wash them with running water, remove the toe bones, and pull out the pig’s hair to obtain a complete pig’s trotter skin, boil it in boiling water for 15 minutes, and then cool it down for later use;
[0039] B. Raw material pretreatment: Wash the fresh and odorless pork lean meat with running water, drain, put it in a meat grinder and grind it into 0.5cm meat filling;
[0040] C. Pickling: Add marinade to the minced meat in step B, stir well, and marinate at 0-4°C for 24 hours, wherein the marinade is composed of the following components by weight: per 100kg of minced meat, salt 3kg, fructose 1kg, compound phosphate 0.2kg, sodium nitrite 0.015kg, ascorbic acid 0.02kg;
[0041] D. Tumbling: add the marinated meat stuffing into the vacuum tumbler, the tumbling conditions are vacuum degree 85...
Embodiment 2
[0048] A method for artificially inoculating and fermenting to produce bound hooves, comprising the following steps:
[0049] A. Pig’s trotter skin preparation: thaw frozen pig’s trotters, wash them with running water, remove the toe bones, and pull out the pig’s hair to obtain a complete pig’s trotter skin. Boil in boiling water for 10 minutes, then cool down and set aside;
[0050] B. Raw material pretreatment: Wash the fresh lean pork meat with no peculiar smell under running water, drain it, put it into a meat grinder and grind it into 0.3cm meat filling;
[0051] C. Pickling: Add marinade to the minced meat in step B, stir evenly, and marinate at 0-4°C for 8 hours. The marinade is composed of the following components by weight: 1.5 salt per 100kg of minced meat. kg, glucose 2kg, compound phosphate 0.5kg, sodium nitrite 0.01kg, ascorbic acid 0.03kg;
[0052] D. Tumbling: Add the marinated meat stuffing into the vacuum tumbler, the tumbling conditions are vacuum degree 80k...
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