Method for quickly detecting vigor of morchella esculenta L. hyphae

Abstract

The invention relates to a method for quickly detecting the vigor of morchella esculenta L. hyphae. The method includes the steps that after a bacterial strain to be detected is cultivated in mother strain flat plate culture media, a bacterium block on a flat plate is cut to be transferred into liquid deep layer culture media for shake culture, and accordingly bacterium liquid is obtained; the bacterium liquid is collected into a centrifuge tube for centrifuging, liquid supernatant is removed, the hyphae are extracted, and the hyphae are washed by normal saline; the hyphae are weighed and added with water to be matched into the bacterium liquid to be detected; the bacterium liquid to be detected, a Tris-HCl buffer solution and a TTC (2,3,5-triphenyltetrazolium chloride) solution are transferred into the centrifuge tube for reacting; after reacting is completed, reacting liquid is placed on ice immediately, centrifuging is conducted at the temperature of 4 DEG C, a cell sediment is obtained, and an extraction agent is added into the centrifuged cell sediment so that extract liquor can be obtained; the extract liquor is centrifuged, the liquid supernatant is collected, and the absorbance value is detected at the wave length of 485 nm. Compared with a traditional method that the vigor of strains is detected according to hypha characteristics, the method has the advantages of being stable and reliable in detection result, good in reproducibility, high in accuracy, convenient to operate, high in speed and the like.

Description

technical field [0001] The invention relates to a method for detecting enzyme activity, more specifically a method for detecting the mycelia activity of edible and medicinal fungi, and belongs to the field of detection of physiologically active substances. Background technique [0002] Morel (Morchella esculenta L.), also known as delicious Morchella, commonly known as goat sparrow, corn fungus, etc., belongs to Ascomycotina (Ascomycotina), Discomycetes (Discomycetes) and Discomycetes ( Pezizales), Morchellaceae, Morchella. It gets its name because its cap is a mesh body full of depressions and ridges, shaped like a morel. Morchella has high nutritional value and medicinal value, and is a large edible and medicinal fungus. According to the determination, the protein content of morel is 22.1%, containing 19 kinds of amino acids, zinc, selenium, iron, germanium, copper and other trace elements and vitamin B 1 , B 2 , B 12 , niacin, pantothenic acid, biotin and other vitam...

AI Technical Summary

Problems solved by technology

[0004] Morel strains, like other edible and medicinal fungi, belong to fungi, with significant genetic variation, excellent traits cannot be completely inherited stably, and are prone to aging or degeneration, which brings great risks to batch cultivation and is also a difficult problem

[0005] How to select an excellent production species is the key to successful cultivation. The traditional method is to select species based on morphological characteristics, physiological and ecological characteristics and cultivation habits, but morel strains require strict external conditions and are significantly affected by external factors, leading to this method. Inaccurate and time consuming

Images