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A polypeptide hydrogel scaffold loaded with drugs and fat-derived mesenchymal stem cells and its preparation method

A fat-derived, stem cell-based technology, applied in drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2017-08-25
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research on the use of polypeptide hydrogel scaffolds to simultaneously load adipose-derived mesenchymal stem cells and the anticancer drug tamoxifen.

Method used

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  • A polypeptide hydrogel scaffold loaded with drugs and fat-derived mesenchymal stem cells and its preparation method
  • A polypeptide hydrogel scaffold loaded with drugs and fat-derived mesenchymal stem cells and its preparation method
  • A polypeptide hydrogel scaffold loaded with drugs and fat-derived mesenchymal stem cells and its preparation method

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Embodiment 1

[0023] Preparation of polypeptide hydrogel scaffolds loaded with drugs and adipose-derived mesenchymal stem cells:

[0024] (1), use ultrapure water to prepare a concentration of 1.836×10 -5 M~2.651×10 -5 M's tamoxifen citrate solution;

[0025] (2), according to Sigma Aldrich HydroMatrix TM Peptide Cell Culture Scaffold Instructions Formed in a 24-well culture plate containing 1.836×10 -5 M~2.651×10 -5 1% polypeptide hydrogel of M tamoxifen citrate;

[0026] Proceed as follows:

[0027] Spreading Cells on Hydrogel Surfaces

[0028] 1. Dilute the stock solution with a concentration of 1% (w / v) to the required concentration with distilled water, and place the resulting working solution on ice before use;

[0029] 2. Add the working solution into the culture well. The recommended volume of the working solution is shown in Table 1. Add 1-2 times the volume of medium along the well wall to start the gel formation;

[0030] Table 1. Recommended volumes of working fluids

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Abstract

The invention discloses a polypeptide hydrogel scaffold loaded with drugs and fat-derived mesenchymal stem cells and a preparation method thereof. (1) Use ultrapure water to prepare a tamoxifen citrate solution with a concentration of 1.836×10‑5M~2.651×10‑5M; (2) form a culture plate containing 1.836×10‑5M~2.651×10‑5M The polypeptide hydrogel of 5M tamoxifen citrate; (3) the tamoxifen citrate solution containing 1.836×10‑5M~2.651×10‑5M in the polypeptide hydrogel in the washing step (2) The pH of the solution was 7.0; (4) Take human adipose-derived mesenchymal stem cells at 1.0×105 cells / ml and add 500 μl to each well. After culturing for 3 days, a polypeptide hydrogel scaffold loaded with drugs and adipose-derived mesenchymal stem cells was obtained. The polypeptide hydrogel scaffold of the present invention contains both the anticancer drug tamoxifen citrate and human adipose-derived mesenchymal stem cells, and can be used to construct living tissues.

Description

[0001] Technical field: [0002] The invention relates to a polypeptide hydrogel scaffold, in particular to a polypeptide hydrogel scaffold loaded with drugs and fat-derived mesenchymal stem cells and a preparation method thereof. [0003] Background technique: [0004] Compared with 2D monolayer cell culture, 3D culture models can better mimic the microenvironment of cells in natural tissues, so this technology is more valuable in studying disease progression and cell response to drugs. Among many materials, the novel scaffold material, which uses polypeptide as the basic unit and relies on the natural self-assembly of polypeptide molecules, has nano-scale fiber composition, simple amino acid composition and sufficient water content, which is closer to the natural extracellular matrix and can provide cells with The ideal three-dimensional environment, and as a biological substance, peptides have good physiological functions such as high bioavailability, low toxicity and specif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/22A61L27/38A61L27/54A61K47/42A61K31/138A61P35/00
Inventor 徐峰伍慧敏
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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