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Dissimilatory iron reducing bacteria and application thereof

A technology of dissimilating iron and bacterial strains, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve problems affecting migration and transformation, and achieve the effect of strong practical application value

Inactive Publication Date: 2015-10-14
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, some Fe(III) reducing bacteria can also reduce toxic heavy metals and metalloids such as Mn(IV), Cr(VI), As(V), thus affecting the migration and transformation of these elements in the natural environment

Method used

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  • Dissimilatory iron reducing bacteria and application thereof
  • Dissimilatory iron reducing bacteria and application thereof
  • Dissimilatory iron reducing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Isolation, screening and identification of bacterial strains

[0018] (1) Material preparation:

[0019] 1. The experimental water samples were taken from the high-arsenic pollution area of ​​Hangjinhou Banner, Hetao Plain, Inner Mongolia.

[0020] 2. Medium:

[0021] IRM liquid medium: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO4 0.6g / L, yeast extract 0.5g / L; add sodium acetate 20mmol / L, iron citrate 20mmol / L, medium pH 6.7; use high-purity nitrogen to deoxygenate, high temperature and high pressure sterilization.

[0022] IRM solid medium: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO4 0.6g / L, yeast extract 0.5g / L, add sodium acetate 20mmol / L, iron citrate 20mmol / L, medium pH 6.7; add 1.8wt% agar, use high-purity nitrogen to deoxygenate, High temperature and high pressure sterilization.

[0023] LB liquid medium: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, distilled water 1000mL, pH 7.0, deoxygenated with high-purity nitrogen,...

Embodiment 2

[0041] Application of Example 2 Klebsiella oxytoca (Klebsiella oxytoca) IMFRCUG-1 in reducing Fe(III)

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Abstract

The invention belongs to the field of environmental biotechnology and particularly relates to dissimilatory iron reducing bacteria and an application thereof. The dissimilatory iron reducing bacteria provided by the invention is Klebsiella oxytoca IMFRCUG-1 which is collected in the China Center for Type Culture Collection on May 27th, 2015, with a collection number CCTCC NO:M2015332. The Klebsiella oxytoca IMFRCUG-1 provided by the invention has a relatively strong reducing function for dissimilatory reduction of Fe(III) and can effectively reduce the Fe(III) in ferric citrate and montmorillonite. Through the invention, the application research idea in the functions of Klebsiella oxytoca is widened, a new material is provided for the control of environmental pollutants by the dissimilatory iron reducing bacteria, and the Klebsiella oxytoca has relatively high value in practical application.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a dissimilating iron-reducing bacterium and its application. Background technique [0002] Iron is the most abundant transition metal element on Earth. The iron cycle process dominated by ferrous iron (Fe(II))-ferric iron (Fe(III)) in the surface environment is considered to regulate the global organic carbon burial, organic pollutants / heavy metal migration and transformation, and biogenic elements. One of the key factors of geological processes such as migration and environmental changes. Microorganisms are the core participants in the iron cycle on the surface, and their action processes are mainly reflected in two ways: assimilation and dissimilation. Among them, dissimilation, which is characterized by iron oxidation or reduction outside the cell, is an important driving force of the iron cycle. [0003] Dissimilatory iron-reducing bacteria refer to a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/22
Inventor 李平王艳红姜舟戴欣月张睿洪文涛
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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