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Method for desulfurization regeneration of waste rubber by mixing two types of microorganism bacteria

A technology for regenerating waste rubber and microbial bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve simple and controllable desulfurization process, good desulfurization effect, and no secondary pollution

Active Publication Date: 2015-10-07
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above research on biological desulfurization is all about the effect of a single strain of bacteria, but there is no systematic report on the effect of the mixed symbiosis process of various microorganisms on the synergistic desulfurization and regeneration of waste rubber.

Method used

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  • Method for desulfurization regeneration of waste rubber by mixing two types of microorganism bacteria
  • Method for desulfurization regeneration of waste rubber by mixing two types of microorganism bacteria
  • Method for desulfurization regeneration of waste rubber by mixing two types of microorganism bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Strains: Sphingomonas, purchased from Shanghai Fuzhong (Apennines) Biotechnology Development Co., Ltd.; Gordonella, purchased from China Industrial Microbiology Culture Collection Management Center.

[0024] Medium A (the medium used when Sphingomonas is cultured alone): NH 4 Cl 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 ·3H 2 O 4g / L, MgSO 4 ·7H 2 O 0.8g / L, CaCl 2 0.01g / L, Na 2 S 2 o 4 ·5H 2 O10g / L, peptone 1g / L, yeast powder 0.1g / L, glucose 2g / L, and the rest is water; medium B (medium used when Gordonella is cultured alone): NH 4 Cl 2g / L, KH 2 PO 4 2.44g / L,Na 2 HPO 4 ·3H2O5.57g / L, MnCl 2 4H 2 O 0.008g / L, MgCl 2 0.2g / L, ZnCl 2 0.001g / L, CoCl 2 ·6H 2 O0.004g / L, CaCl 2 0.04g / L,AlCl 3 ·6H 2 O 0.001g / L, CuCl 2 2H 2 O,0.001g / L,FeCl 3 ·7H 2 O 0.04g / L, H 3 BO 3 0.001g / L,NaMoO 4 2H 2 O 0.001g / L, glucose 4g / L, and the rest is water.

[0025] Exchange medium culture: prepare 100mL medium A and 100mL medium B in 250mL shake flasks, and sterilize at 115°C f...

Embodiment 2

[0028] Strains: Sphingomonas, purchased from Shanghai Fuzhong (Apennines) Biotechnology Development Co., Ltd.; Gordonella, purchased from China Industrial Microbiology Culture Collection Management Center.

[0029] Rubber: styrene-butadiene rubber vulcanized rubber filled with 30 parts by weight of carbon black, cut into 1cm×1cm film. The specific formula is as follows: styrene-butadiene rubber raw rubber 100 (parts by weight), zinc oxide 4, stearic acid 2, accelerator D 0.6, accelerator DM 1.2, sulfur 1.8, antioxidant RD 1.0, carbon black 30.

[0030] Medium: NH 4 Cl 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 ·3H 2 O 4g / L, MgSO 4·7H 2 O0.8g / L, CaCl 2 0.01g / L, Na 2 S 2 o 4 ·5H 2 O 10g / L, peptone 1g / L, yeast powder 0.1g / L, glucose 2g / L, and the rest is water.

[0031] Co-cultivation desulfurization process: soak styrene-butadiene rubber vulcanizate film in ethanol with a mass percentage concentration of 75% for 24 hours for detoxification; prepare 100mL medium in a 250mL sh...

Embodiment 3

[0034] Strains: Sphingomonas, purchased from Shanghai Fuzhong (Apennines) Biotechnology Development Co., Ltd.; Gordonella, purchased from China Industrial Microbiology Culture Collection Management Center.

[0035] Rubber: styrene-butadiene rubber vulcanized rubber filled with 30 parts by weight of carbon black, cut into 1cm×1cm film. The specific formula is as follows: styrene-butadiene rubber raw rubber 100 (parts by weight), zinc oxide 4, stearic acid 2, accelerator D 0.6, accelerator DM 1.2, sulfur 1.8, antioxidant RD 1.0, carbon black 30.

[0036] Medium: NH 4 Cl 0.4g / L, KH 2 PO 4 4g / L, K 2 HPO 4 ·3H 2 O 4g / L, MgSO 4 ·7H 2 O0.8g / L, CaCl 2 0.01g / L, Na 2 S 2 o 4 ·5H 2 O 10g / L, peptone 1g / L, yeast powder 0.1g / L, glucose 2g / L, and the rest is water.

[0037] Co-cultivation desulfurization process: soak styrene-butadiene rubber vulcanizate film in ethanol with a mass percentage concentration of 75% for 24 hours for detoxification; prepare 100mL medium in a 250mL s...

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Abstract

The invention provides a method for desulfurization regeneration of waste rubber by mixing two types of microorganism bacteria and relates to a desulfurization regeneration technology of the waste rubber. According to the method, an enzyme generated in the mixed growth process of sphingomonas sp. and gordonia sp. is used for performing desulfurization regeneration on the waste rubber. An early study shows that the sphingomonas sp. or the gordonia sp. has a good desulfurization effect on waste tread rubber, styrene-butadiene rubber and natural rubber, and in order to study mixed bacterial desulfurization, the system provided by the invention adjusts a culture medium for symbiosis of the two bacteria, and the two bacteria are mixed together to perform desulfurization regeneration on the waste rubber. A study finds that during co-culture desulfurization, the biomass of the mixture of the two types of bacteria is higher than that of a single strain, which shows that the two bacteria have a synergistic effect. According to the method provided by the invention, the inoculation means of the two types of bacteria and the influence of process conditions for growth and metabolism of the two types of bacterial on the desulfurization of the waste rubber are studied, so that a best process for co-culture desulfurization is determined. Compared with undesulfured waste rubber, the desulfurized rubber is increased in selling value, lowered in surface sulfur element content, and increased in oxygen content. Therefore, the mixing of the two types of the microorganism bacteria has a relatively obvious desulfurization effect on the rubber surface.

Description

technical field [0001] The invention relates to a desulfurization regeneration technology of waste rubber, in particular to a method and a process for mixing two kinds of microbial bacteria to desulfurize waste rubber. Background technique [0002] With the rapid development of the world's industry, the consumption of rubber products is gradually increasing around the world, and my country has also rapidly developed into a big country in the world's rubber industry. At the same time, the waste of rubber products is also increasing. It is reported that 1 million tons of waste rubber products are accumulated around the world every year, and waste tires alone account for 60%-70% of all waste rubber products. . However, due to the stable three-dimensional network structure of rubber products, they cannot be degraded in the natural environment for a long time, resulting in black pollution of the ecological environment and serious waste of resources. Therefore, the effective rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P1/04C12R1/01
Inventor 赵素合崔肖肖王炳武胡明翰李超
Owner BEIJING UNIV OF CHEM TECH
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