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Preparation and application of a bacterial strain resistant to botrytis cinerea and antagonist

A technology for vegetables, fruits and antagonists, which can be used in applications, fungicides, bacteria, etc., can solve the problems of chemical pesticides polluting the environment, endangering human health and life, etc., and achieves the effects of stable formulations, abundant spores, and easy industrialization and amplification

Inactive Publication Date: 2018-08-10
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical pesticides seriously pollute the environment, and the pesticides left on agricultural products endanger human health and life

Method used

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  • Preparation and application of a bacterial strain resistant to botrytis cinerea and antagonist
  • Preparation and application of a bacterial strain resistant to botrytis cinerea and antagonist

Examples

Experimental program
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Effect test

Embodiment Construction

[0028] 1. Strains and screening

[0029] 1. Preparation of fermented crude extract

[0030] Eighty-seven spore-forming actinomycetes isolated from the soil of Ailaoshan National Nature Reserve in Yunnan were inoculated on the ISP2 agar slant. The size of the slant test tube was ø15×150 mm, and the volume of the slant medium was 5 mL. Each strain was inoculated with 2 slopes, one of which was a spare slope, and cultured at 28°C for 4-7 days. After the mycelia had formed a large number of spores, in addition to the spare slope, 5 mL of sterile deionized water was added to the slope, and sterilized Scrape and wash the bacterial lawn lightly with a bamboo stick to prepare a spore suspension for later use.

[0031] The preparation method of the above slant medium is: ISP2 medium (g / L): 4.0 g of yeast extract, 10.0 g of malt extract, 4.0 g of glucose, 20.0 g of agar, 1 L of tap water; natural pH; Bacteria for 20 min.

[0032] The spore suspension of each strain was inoculated int...

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Abstract

The invention discloses a strain resistant to gray mold of vegetables and fruits, preparation and application of an antagonist. The microbial strain is Streptomyces.sp strain ALS-045, and the preservation number is CGMCC No: 10986. Antagonist preparation includes strain screening, greenhouse pot activity verification, strain biological identification, preparation of biologically active preparations, field activity verification and other steps. The bacterial strain ALS-045 of the present invention is an excellent material for biological preparations for preventing and treating Botrytis cinerea disease in vegetables and fruits, has abundant spore production, simple liquid culture conditions for the strain, stable dosage form, easy industrial scale-up, and excellent potential for being developed as a green biological pesticide.

Description

technical field [0001] The invention belongs to actinomycetes strains and preparations for antagonizing vegetable and fruit pathogens, in particular to streptomyces ( Streptomyces . sp) strains and their antagonistic preparations. Background technique [0002] Botrytis cinerea is a fungal disease with a wide range of hosts. It commonly occurs and is popular in greenhouse cultivation of fruits and vegetables. Botrytis cinerea is caused by fungi of the genus Botrytis of the family Hyphospora cause, infection Botrytis cinerea Caused by the bacterium Botrytis cinerea. The spore peduncle of the fungus is clustered with several roots, brown, with a septum, and the top is 1-2 times branched, and the top is densely populated with small stalks, and the size is 1452.5-3168.2 microns × 8.5-11.5 microns. Conidia are oval to round, unicellular, nearly colorless, with a size of 4.2-10.5 microns × 3.5-7.5 microns. [0003] Botrytis cinerea mainly survives the summer with diseased my...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 文孟良李铭刚韩秀林赵江源王永霞
Owner YUNNAN UNIV
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