A Pseudomonas maltophilia strain qba-5 with inhibitory effect on Botrytis cinerea and its application
A technology of Pseudomonas and Botrytis cinerea, applied in the field of microorganisms, can solve problems affecting the sustainable and healthy development of agriculture, excessive pesticide residues, environmental pollution, etc., and achieve good market application prospects
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[0020] 1. Isolation and screening of strains
[0021] On March 19, 2013, 20 tomato leaf samples were collected in a greenhouse in Laiyang City. Each leaf was soaked in 75% alcohol for 10 seconds, then soaked in 0.1% mercury chloride for 30 seconds, rinsed with sterile water for 3 times, and transferred to sterilized water. Grind in a mortar treated with bacteria, add sterile water to dilute to 10ml, continue to dilute to 10ml with sterile water -5 times, take 100ul respectively and apply LB plates (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, pH 7.0), and culture at 30°C.
[0022] Pick a single colony and inoculate it in YEPD medium (yeast extract 3 g / L, peptone 10 g / L, glucose 20 g / L) at 25°C and 120 rpm for 24 hours, then culture at 25°C and 12000 rpm Centrifuge under the conditions, filter the supernatant through a filter with a diameter of 0.22um, inoculate the conidia of Botrytis cinerea into the filtrate and let it stand for cultivation, inoculate the co...
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