Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for simultaneous preparation of foliar and root-applied agricultural biocontrol preparations and its application

A technology of agricultural production and preparation, applied in the biological field, can solve the problems of nematocide use restriction, chemical pesticide-free, etc., and achieve the effect of saving equipment investment, reducing production cost, and easy cultivation

Active Publication Date: 2019-12-17
宁夏张裕龙谕酒庄有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control of plant parasitic nematodes usually relies on chemical nematicides, cultivation techniques, breeding resistant varieties and biological pesticides, but there are still no specific chemical pesticides, and due to potential environmental problems, involving human and animal health, the use of nematicides subject to great restrictions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for simultaneous preparation of foliar and root-applied agricultural biocontrol preparations and its application
  • A method for simultaneous preparation of foliar and root-applied agricultural biocontrol preparations and its application
  • A method for simultaneous preparation of foliar and root-applied agricultural biocontrol preparations and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Shake flask culture simultaneously prepares foliage and root application agricultural biocontrol preparations

[0030] Pick one ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 30°C and 180rpm for 32h until the bacteria become turbid to obtain the activated seed solution. Activation medium (g / L): 3 peptones, 1 yeast powder, 10 glucose, 2 powdered chitosan, 1 potassium dihydrogen phosphate, 1 magnesium sulfate, 1 disodium hydrogen phosphate, pH 7.0. Pack in a 500ml Erlenmeyer flask 100ml activation medium, sterilized at 121°C for 20min.

[0031] Use the preferred medium (g / L): glycerol 18, soybean peptone 25, MnSO 4 1, powdered chitosan (deacetylation degree above 90%) 20, crab shell powder 10, NaCl 2, CaCl 2 1. MgSO 4 1. FeSO 4 0.5, pH 5, divide 200ml of fermentation medium into each 500ml Erlenmeyer flask, and sterilize at 121°C for 20min. According to the inoculum amount of 0....

Embodiment 2

[0033] Example 2 Fermentation tank (10L) culture simultaneously prepares foliage and root application agricultural biocontrol preparations

[0034] Activation medium (g / L): peptone 5, yeast powder 3, glucose 20, chitosan powder 5, potassium dihydrogen phosphate 3, magnesium sulfate 2, disodium hydrogen phosphate 3, pH 7.0. Pack in a 500ml Erlenmeyer flask 100ml activation medium, sterilized at 121°C for 20min. Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 32°C and 180 rpm for 36 hours until the bacteria become turbid to obtain the activated seed solution.

[0035] Use the preferred medium (g / L): glycerol 30, soybean peptone 40, MnSO 4 3. Powder chitosan (over 90% degree of deacetylation) 30, crab shell powder 15, NaCl 5, CaCl 2 3. MgSO 4 3. FeSO 4 1.5. Fill the fermentation medium with a ratio of 70%, and the liquid volume is 7L. After steam sterilization at 118°C for 20 minutes, inser...

Embodiment 3

[0037] Example 3 Fermentation tank (50L) culture simultaneously prepares foliage and root application agricultural biocontrol preparations

[0038] Activation medium (g / L): peptone 4, yeast powder 2, glucose 15, chitosan powder 3, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 2, pH 7.0. Pack in 500ml Erlenmeyer flask 100ml activation medium, sterilized at 121°C for 20min. Pick one ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 30°C and 180 rpm for 34 h until the bacteria become turbid to obtain the activated seed solution.

[0039] Use the preferred medium (g / L): glycerol 20, soybean peptone 30, MnSO 4 2. Powder chitosan (deacetylation degree above 90%) 25. Crab shell powder 12. NaCl 4. CaCl 2 2. MgSO 4 2. FeSO 4 1. Fill the fermentation medium with a proportion of 70%, and the liquid volume is 35L. After steam sterilization at 118°C for 20 minutes, insert ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a simultaneous preparation method and application of agricultural biocontrol preparations for leaves and roots. The product is obtained by liquid fermentation of Paecilomyces lilacinus YT08 with a preservation number of CGMCC No. 10026. After the fermentation is completed, The fermentation liquid is separated from solid and liquid, and the solid part containing the bacteria is directly used as the agricultural biocontrol agent for root application, while the liquid part is the agricultural biocontrol agent for foliage. The preparation method of the invention is simple, the production process is easy to control, and is suitable for large-scale production in factories, and the foliage and root application agricultural biocontrol preparations have strong inhibitory effects on plant pathogenic bacteria and root-knot nematodes respectively.

Description

technical field [0001] The invention relates to a method for simultaneously preparing agricultural biocontrol preparations for foliar application and root application, and its application in biological control of crop pathogens and root-knot nematodes, belonging to the field of biotechnology. Background technique [0002] Plant nematode disease is one of the four major pathogenic organisms in the cultivation of crops, vegetables, fruit trees, forest trees, flowers and medicinal materials. Its harm is second only to fungi and surpasses bacteria and viruses. Plant parasitic nematodes cause about 100 billion US dollars of losses in world agricultural production every year. The most important nematodes in global agriculture are root-knot nematodes and cyst nematodes. The more serious nematodes in my country include root-knot nematodes, soybean cyst nematodes, and wheat grain nematodes. , sweet potato stem nematode, rice stem apex nematode, chestnut nematode, pine wood nematode, e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12N1/14A01N63/04A01P3/00A01P5/00C12R1/79
CPCA01N63/30C12N1/14C12P1/02C12N1/145C12R2001/79
Inventor 程仕伟解孝满张萍缪静刘丹李加文
Owner 宁夏张裕龙谕酒庄有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com