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Analysis method for content of paeonol based on chromatograph-spectrograph combination and sub-space included angle criteria

A technology of subspace angle and analysis method, which is applied in the analysis field of paeonol content, can solve the problems of high analysis cost, low analysis efficiency, and high operation intensity, and achieve practical quantitative analysis technology, simple operation, stable and reliable method Effect

Inactive Publication Date: 2015-09-30
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of analysis method of paeonol content based on chromatograph-spectrometer coupling and subspace angle criterion, solve the problem of low analysis efficiency, high operation intensity and high analysis cost existing in the prior art

Method used

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  • Analysis method for content of paeonol based on chromatograph-spectrograph combination and sub-space included angle criteria
  • Analysis method for content of paeonol based on chromatograph-spectrograph combination and sub-space included angle criteria
  • Analysis method for content of paeonol based on chromatograph-spectrograph combination and sub-space included angle criteria

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Experimental program
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Effect test

preparation example Construction

[0032] Step 2, the preparation of the sample to be tested and the collection of ultraviolet spectrum,

[0033] Take the mother liquor of paeonol crystallization, at room temperature, take about 0.15g of the upper layer solution and one portion of the lower layer crystal, and dilute with 75% ethanol to a concentration of 10-20mg / L to obtain sample S 1 and S 2 .

[0034] Take two parts of paeonol crystallization mother liquor from different production batches, heat them to 50°C respectively to melt, weigh 1.2g and 0.9g respectively, and dilute to 10-20mg / L with 75% ethanol to obtain sample S 3 and S 4 .

[0035] Weigh 0.1g of paeonol crystalline product, dilute to 10-20mg / L with 75% ethanol, obtain sample S 5 ; take S 1 -S 5 Equal volumes were mixed to obtain sample S 6 .

[0036] Collection S 1 -S 6 The ultraviolet spectrum data of the sample to be tested is obtained, which is denoted as D. The integration time is 15 microseconds; the number of integrations is 20 tim...

Embodiment 1

[0051] 1 Experimental part

[0052] 1.1 Instruments and reagents

[0053] High-performance liquid chromatography (LC-20AT, Shimadzu); UV-visible fiber optic spectrometer (Maya2000, Ocean Optics); electronic analytical balance (AL104, Mettler-Toledo); ultrasonic cleaning machine (Shanghai Zhixin Instrument Co., Ltd. ); absolute ethanol (AR), methanol (chromatographically pure), acetonitrile (chromatographically pure), paeonol reference substance (purity 99.9%), paeonol crystallization mother liquor (Guangxi Yikang Pharmaceutical Industry Co., Ltd.).

[0054] 1.2 Test method

[0055] 1.2.1 Establishment of paeonol standard spectral library

[0056] Accurately weigh 0.1010g paeonol reference substance, and dilute to 100mL with 75% ethanol. Prepare successively the concentration of 2mg / L, 4mg / L, 8mg / L, 12mg / L, 16mg / L, 20mg / L paeonol-75% ethanol solution B 1 -B 6 . The 200nm-400nm ultraviolet spectrum of each solution was collected, and the standard spectral library V was obt...

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Abstract

The invention discloses an analysis method for the content of paeonol based on chromatograph-spectrograph combination and sub-space included angle criteria. The analysis method is specifically carried out according to the following steps: establishing a paeonol standard spectrum base; preparing a sample to be detected and acquiring an ultraviolet spectrum; establishing a modeling background database; and determining the content of paeonol in the sample to be detected. According to the invention, the rapid analysis method for the content of paeonol is established by the liquid phase-ultraviolet visible spectrograph combination and the application of the sub-space included angle criteria. With regard to the determination of similar products, samples can be detected in a large batch by one modeling. Compared with a high performance liquid chromatography, the established method is stable and reliable and is simple and convenient to operate; the samples do not need to be subjected to complicated pre-treatment; and a relatively practical quantitative analysis technology is provided for rapidly determining the content of paeonol and can be popularized and applied to other paeonol product detection fields.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to an analysis method for paeonol content based on the combined use of a chromatography-spectrometer and a subspace angle criterion. Background technique [0002] Paeonol is a traditional Chinese medicine extracted from the root bark of the national flower of China, peony. Extracted from the dried root bark of the ranunculaceae tree peony. Paeonol has a wide range of pharmacological applications. In addition to being used as raw materials for medical preparations, it is also used in toothpaste, skin care, beauty and other daily chemicals. The content determination of paeonol is one of the main indicators for the quality control of various preparations and products. At present, high performance liquid chromatography is mostly used for the analysis of paeonol content. This method has high sensitivity, but the analysis cost is high, the operation intensity is high, ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 粟晖姚志湘闫一夫方凤刘柳
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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