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Application of puerarin to preparing pain easing medicine

A technology of puerarin and medicine, applied in the field of pharmaceuticals, to achieve powerful analgesic effects, reduce adverse reactions, and rich sources

Inactive Publication Date: 2015-09-30
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there is no report about using puerarin in drugs that enhance the analgesic effect of opioids in the prior art, nor is there any report about using puerarin in drugs that delay or reduce opioid tolerance

Method used

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  • Application of puerarin to preparing pain easing medicine
  • Application of puerarin to preparing pain easing medicine
  • Application of puerarin to preparing pain easing medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 (acute analgesic effect enhancement experiment)

[0030] a) Experimental animals: healthy adult female ICR mice, clean grade, weighing 18-22 g. The experimental animals were raised in an independent environment with 12h-12h alternation of day and night, the room temperature was maintained at 24±2°C, water and food were free to drink, and the experiment was carried out after 1 week of adaptation to the environment. All handling of animals followed the requirements of the Ethics Committee of the International Association for the Study of Pain.

[0031] b) Test drugs and reagents:

[0032] The drug puerarin, with a purity of 98%, is commercially available. Morphine hydrochloride was purchased from Shenyang No. 1 Pharmaceutical Factory.

[0033] c) Test method:

[0034] Dosage and method of administration of puerarin and morphine: puerarin was dissolved in physiological saline, and the administration dose was 60 mg / kg, and the administration methods were al...

Embodiment 2

[0039] Example 2 (Opioid Tolerance Alleviation Experiment)

[0040] a) Test animals:

[0041] Female ICR mice, clean grade, weighing 18-22 g. The experimental animals were raised in an independent environment with 12h to 12h alternation of day and night, the room temperature was maintained at 24±2°C, and they had free access to water and food. Experiments were carried out after 1 week of adaptation to the environment. All handling of animals followed the requirements of the Ethics Committee of the International Association for the Study of Pain.

[0042] b) Test drugs and reagents:

[0043] Drug Puerarin, 98% pure, commercially available. Morphine hydrochloride was purchased from Shenyang No. 1 Pharmaceutical Factory.

[0044] c) Test method:

[0045] Dosage and method of puerarin administration: puerarin was dissolved in physiological saline, the dose was 60 mg / kg, and the administration method was intragastric administration.

[0046] ICR mice were randomly divided int...

Embodiment 3

[0050] Embodiment 3 (inhibition of inflammatory factor mRNA up-regulation experiment)

[0051] a) Test cells:

[0052] BV2 microglial cells were cultured in DMEM medium containing 10% fetal bovine serum, 5% CO2, and cultured in a constant temperature incubator at 37°C. It can be subcultured when it covers 80% of the bottom of the culture bottle. 24 hours before the experiment, the medium was replaced with 0.5% fetal bovine serum high glucose DMEM.

[0053] b) Test drugs and reagents:

[0054] Fetal bovine serum was purchased from Hyclone; DMEM and Trizol were purchased from Invitrogen; TaKaRa PrimeScript kit was purchased from Dalian Bao Biological Engineering Co., Ltd.

[0055] c) Test method:

[0056] Real-time quantitative PCR: the RNA of each group was extracted by Trizol method. SYBR Green Assay of TaKaRa PrimeScript kit was used for reverse transcription reaction. The reaction system was: 5×PrimeScript Buffer 2μl, Prime Script RT Enzyme 0.5μl, Oligo dT Primer 0.5μl,...

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Abstract

The invention discloses new application of puerarin to preparing a pain easing medicine, and particularly discloses the application of the puerarin to preparing the medicine for strengthening the opioid medicine pain easing effect and delaying or alleviating the opioid tolerance. When the puerarin is matched with an opioid medicine to be used, or the puerarin and the opioid medicine are prepared into a compound preparation to be used, the pain easing time of the medicine can be prolonged, the pain easing efficiency can be strengthened, and the opioid tolerance in the opioid medicine treatment process can be further delayed or alleviated. The effect is specifically achieved by restraining spinal glial cell overactivity induced by the opioid medicine through the puerarin.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to the new application of puerarin in the field of analgesic drugs, in particular to the application in the preparation of drugs for enhancing the analgesic effect of opioids and delaying or alleviating opioid tolerance. Background technique [0002] Pain is a complex physiological and psychological activity, including the pain sensation caused by noxious stimuli acting on the body, and the body's pain response to noxious stimuli. Pain can be used as a warning that the body is injured, causing a series of defensive protective reactions of the body, but some acute or long-term severe pain is an unbearable torture to the body. Pain is one of the most common clinical symptoms, and it is also an important problem that medical workers need to solve urgently. [0003] In terms of the course of the disease, pain can be divided into acute pain and chronic pain. Acute pain is pain that is new and of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K9/00A61K45/00A61K31/485A61K9/08A61P25/04
Inventor 季浩刘文涛阚建伟窦长清刘佳
Owner JIANGSU TIANSHENG PHARMA
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