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Aspergillus aculeatus strain and application thereof

A technology of Aspergillus aculeatus and strains, applied in the fields of fermentation engineering and biology, can solve the problem of low activity of β-glucosidase, and achieve the effects of reasonable design, easy process control and high enzyme activity

Inactive Publication Date: 2015-09-09
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] In the prior art, the extracellular β-glucosidase activity of Aspergillus aculeatus strains producing β-glucosidase is not high, and the inventors of the present application obtained a strain of Aspergillus aculeatus with higher extracellular β-glucosidase activity

Method used

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  • Aspergillus aculeatus strain and application thereof

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Embodiment Construction

[0022] The technical solution of the present invention will be further specifically described below through specific examples.

[0023] 1. Isolation and purification of bacterial strains

[0024] 1.1 Sample collection: 25 soil samples were collected in sterile test tubes from the soil under drainage ditches of small bamboo forests, wild tea trees, rotten bamboo leaves and dead peach trees.

[0025] 1.2 Sample processing

[0026] Reagent preparation: sample treatment solution containing antibiotics configuration: ampicillin 1g, kanamycin 0.55g, sodium chloride 8.5g, Tween 1g dissolved in appropriate amount of water; 1g chloramphenicol dissolved in 4.5ml ethanol; ethanol-chlorine The mycin solution was mixed into the solution obtained in the first step, and the volume was adjusted to 1 L to obtain a treatment solution.

[0027] Screening medium: Caspian medium containing methyl red (g / L): CMC-Na 15.0, sodium nitrate 3.0g, magnesium sulfate heptahydrate 0.5g, potassium chloride...

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Abstract

The invention relates to an aspergillus aculeatus strain and application thereof, and belongs to the technical field of fermentation engineering and biology. The preservation name of the aspergillus aculeatus strain is (Aspergillus sp.) BG30, the preservation department is China microorganism culture preservation management committee common microorganism center, the preservation data is June 28th, 2013, and the preservation number is CGMCC NO.7799. During fermentation of the aspergillus aculeatus strain, the enzymatic activity of ectoenzyme beta-glucosidase is high, by-products are few, the beta-glucosidase is prone to separation and purification, the separation and purification cost is low, and therefore the fact that the aspergillus aculeatus strain serves as original strain to ferment and produce beta-glucosidase facilitates industrial production.

Description

technical field [0001] The invention relates to an Aspergillus aculeatus strain and an application thereof, belonging to the fields of fermentation engineering and biotechnology. Background technique [0002] β-Glucosidase (β-Glucosidase, EC 3.2.1.21), also known as β-D-glucoside glucohydrolase, can hydrolyze the non-reducing β-D-glucosidic bond bound to the terminal and release β -D-glucose and corresponding ligands, this enzyme exists in many plants, and also exists in some yeasts, molds, Trichoderma, bacteria and even insects. β-glucosidase can hydrolyze the flavor precursor β-glucoside in fruits, vegetables and tea into aroma substances with strong natural flavor. [0003] β-glucosidase can be used in a wide range of fields: it can be used in the fruit juice industry for flavor enhancement and debitterness. Many fruit juices contain a lot of flavor precursors - β-glucosides. As long as these glycosides can be combined through the action of some enzymes Base volatilizat...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42C12R1/66
CPCC12N9/2445C12Y302/01021C12N1/145C12R2001/66
Inventor 袁辉
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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