Compound porcine alpha interferon gene and synthetic method thereof

Abstract

The invention discloses a compound porcine alpha interferon gene. Compared with a yeast expressed natural porcine alpha interferon gene, the compound porcine alpha interferon gene has the advantages that the expressed protein of the compound porcine alpha interferon gene is better in antiviral activity, and the function of resisting 100TCID50VSV virus infection on a porcine kidney cell is improved by 50 times. The invention further provides a synthetic method of the compound porcine alpha interferon gene. The synthetic method adopts pichia pastoris X-33 as a template, requires no additional inducer, and is suitable for industrial production. Due to the adoption of pichia pastoris, toxins which are carried by escherichia coli expressed foreign protein and toxic to porcine cells can be avoided, that is, the toxicity is reduced. The synthetic method is suitable for synthesis of the compound porcine alpha interferon gene.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and relates to a gene and a synthesis method thereof, in particular to a compound pig interferon alpha gene and a synthesis method thereof. Background technique [0002] Interferon is mainly produced by animal cells under the action of inducers such as viruses. Many animals, including humans, mammals, birds, fish, etc., can produce interferon. According to the antigenic properties and molecular structure of interferon, it is divided into different types. Interferon (IFN) is divided into type I and type II. Type I interferon gene lacks introns, including 5 types of α, β, δ, ω, and τ. Type II has only one type γ, which contains introns . They have related structures and share the same receptor (primary receptor), which is widely distributed, including monocytes / macrophages, polymorphonuclear leukocytes, B cells, T cells, platelets, epithelial cells, endothelial cells cells and tumor cells. E...

AI Technical Summary

Problems solved by technology

The natural porcine IFN-α induced by inducers is expensive due to many reasons such as few sources, high cost, and complicated purification process, which greatly limits the clinical and scientific applications. System, so that it can be efficiently expressed, and the production of interferon with the advantages of low cost, broad-spectrum antiviral activity, non-toxicity, and no residue is undoubtedly an effective way to promote the application of interferon in veterinary clinics

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