Method for preparing myofibrillar protein-chitosan nano microspheres composite membrane
A myofibrillar protein and chitosan nanotechnology, which is applied in the field of food quality and safety, can solve the problems of minced meat and fish bones not being effectively utilized, restricting the in-depth development of industries, wasting fishery resources, etc., and achieve the purpose of inhibiting food lipids Effect of peroxidation, significant antibacterial activity, and increased added value
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[0018] (1) Preparation of myofibrillar protein from minced tuna: take minced tuna, add Tris-Maleate buffer (0.05 mol / L, pH 7.5) at a material-to-liquid ratio of 1 g:9 mL, and homogenize at 18000 r / min Afterwards, centrifuge at 6000 r / min for 12 min, take the precipitate and repeat homogenization-centrifugation twice, and add 0.1 mol / L NaCl solution to wash the obtained precipitate according to the solid-liquid ratio of 1 g:4.5 mL, and centrifuge at 9000 r / min for 10 min. The obtained precipitate was washed by adding 0.1 mol / L NaCl solution according to the solid-liquid ratio of 1 g:9 mL, filtered through gauze, and the filtrate was centrifuged to obtain a precipitate, which was the myofibrillar protein of tuna mince.
[0019] Preparation of tuna bone gelatin plasticizer: take tuna bone and crush it, add 0.5 mol / L NaOH solution at 4°C for 2.5 h at a material-to-liquid ratio of 1 g:12 mL, wash until neutral Add 0.5 mol / L EDTA solution to 1 g:8 mL, soak at 4 °C for 4 days to deca...
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