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Preparation method for low-calorie structure lipid containing conjugated linoleic acid and application

A technology of conjugated linoleic acid and structural lipids, which can be used in fermentation and other directions to solve problems such as constipation

Active Publication Date: 2015-08-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the saturated fatty acids used in these low-calorie structural fats form fatty acid soaps in the human body, which may cause some side effects such as constipation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 15.1g tributyrin and 24.7g conjugated ethyl linoleate in a 100mL round bottom flask, add 3.98g (10% based on substrate) of lipase Novzyme 435, in a constant temperature water bath at 60°C, The reaction was carried out for 2 hours under the conditions of a vacuum degree of 0.01 MP and a stirring speed of 200 r / min. After the reaction, the lipase was removed by high-speed centrifugation. At the same time, the conjugated ethyl linoleate in the mixture is recovered by molecular distillation to obtain the structured lipid. It is detected that under this condition, the conversion rate of tributyrin is 100%, the content of conjugated linoleic acid in the obtained structured lipid product is 34.1%, and the content of butyric acid is 47.1%. The content of triglyceride (TTC) containing two molecules of butyric acid and one molecule of conjugated linoleic acid is 61.2%, and the content of triglyceride (TCC) containing one molecule of butyric acid and two molecules of conjug...

Embodiment 2

[0026] Weigh 15.1g of tributyrin and 30.8g of ethyl conjugated linoleate in a 100mL round bottom flask, add 3.67g (8% based on substrate) of lipase Novzyme 435, in a constant temperature water bath at 50°C, The reaction was carried out for 2.5 h under the conditions of a vacuum degree of 0.01 MP and a stirring speed of 150 r / min. After the reaction, the lipase was removed by high-speed centrifugation. At the same time, the conjugated ethyl linoleate in the mixture is recovered by molecular distillation to obtain the structured lipid. After detection, under this condition, the conversion rate of tributyrin is 100%, the content of conjugated linoleic acid in the resulting structured lipid product is 41.9%, the content of butyric acid is 37.9%, and the triglyceride consists of The content of TTC is 42.3%, the content of TCC is 29.2%, and the content of CCC is 3.4%.

Embodiment 3

[0028] Weigh 20.0g tributyrin and 33.5g conjugated linoleic acid in a 100mL round bottom flask, add 6.42g (12% based on substrate) lipase Novzyme 435, in a constant temperature water bath at 55 degrees, under vacuum The temperature was 0.01MP and the stirring speed was 250r / min for 2.5h. After the reaction, the lipase was removed by high-speed centrifugation. At the same time, the conjugated linoleic acid in the mixture is recovered by molecular distillation to obtain the structured lipid. After detection, under this condition, the conversion rate of tributyrin is 100%, the content of conjugated linoleic acid in the resulting structured lipid product is 37.3%, the content of butyric acid is 41.4%, and the triglyceride consists of The content of TTC is 51.3%, the content of TCC is 21.6%, and the content of CCC is 2.5%.

[0029] The conjugated linoleic acid obtained above is a class of octadecadienoic acid containing cis or trans conjugated double bonds at the 9 and 11, 10 and...

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Abstract

The invention relates to a preparation method for low-calorie structure lipid containing conjugated linoleic acid and application; the preparation method comprises the following steps: mixing tributyrin with conjugated linoleic acid or ethyl ester thereof to obtain the first mixture; performing contact between the first mixture and the lipase, at certain reaction temperature, vacuum degree and blending condition, performing the reaction for a period of time, and taking the percent conversion of the tributyrin as the index to obtain the second mixture; removing lipase in the reactant, distilling and recovering conjugated linoleic acid or ethyl ester thereof in the second mixture and obtaining the structure lipid containing conjugated linoleic acid. The product prepared by the preparation method is the structure lipid having low-calorie and related function of conjugated linoleic acid.

Description

technical field [0001] The invention relates to the technical field of functional lipids, in particular to a preparation method and application of a low-calorie structured lipid containing conjugated linoleic acid. Background technique [0002] With the improvement of people's living standards, obesity, cardiovascular disease, heart disease, cancer, fatty liver, etc. caused by obesity are gradually perplexing people's lives. Statistics show that for every 5kg body weight increase, the risk of coronary heart disease will increase by 14%, the risk of stroke will increase by 4%, and the risk of ischemic stroke will increase by 16%. The World Health Organization (WHO) has warned that overweight and obesity are the fifth leading risk of death globally. Therefore, the development of some functional oils that can reduce blood fat and visceral fat after eating, and can meet people's requirements for oil flavor, taste and processing suitability will prevent and alleviate obesity, ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6427
Inventor 邹孝强金青哲王兴国黄健花刘睿杰常明王小三潘秋琴
Owner JIANGNAN UNIV
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