A kind of paraffin section method of plant tissue

Abstract

The invention relates to the field of plants, in particular to a method for paraffin sectioning plant tissues. In this method, after the buckwheat peel is soaked in warm water, the peel tissue contains 20% to 40% water, and then the "water-containing" embedding method is used to wrap the water-containing tissue with paraffin. The "water-containing" embedding method can replace the conventional method. The steps of dehydration, transparency, and wax dipping of paraffin sections, and at the same time use a dissecting needle to peel off the paraffin instead of dewaxing steps, greatly reducing the time and operation of the sectioning process. The method cuts out buckwheat pericarp tissue slices with a thickness of 10-16 μm, the tissue is complete, each part is clearly displayed, and the slice and observation effects are obviously enhanced.

Description

technical field [0001] The invention relates to the field of plants, in particular to a method for paraffin sectioning plant tissues. Background technique [0002] Paraffin section is the most widely used method in conventional histological preparation techniques. It is mainly used to observe the morphology of cells and tissues. After being sealed, the prepared slides can be made into permanent slides and stored for a long time. [0003] Conventional procedures for making paraffin sections include steps such as fixation, dehydration, transparency, wax immersion, embedding, and section staining, involving many procedures and links and a long cycle. Moreover, the reagents, concentrations, and time used by different tissue materials are different, and problems such as slice bending and tissue fragmentation are prone to occur during the production process, resulting in failure of production. [0004] The reasons for the failure of the production can be traced back to many defic...

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Problems solved by technology

[0003] Conventional procedures for making paraffin sections include steps such as fixation, dehydration, transparency, wax immersion, embedding, and section staining. There are many procedures and links involved, and the cycle is long.

In addition, the reagents, concentrations, and time used for different tissue materials are different, and problems such as slice bending and tissue fragmentation are prone to occur during the production process, resulting in failure of production

[0004] The reasons for the failure of the production can be traced back to many deficiencies in the production process of paraffin sections, including dehydration, transparency, wax dipping, embedding, sectioning, dewaxing and sealing, etc. If the dehydration is not complete, it will cause transparency and opacity. Thoroughly, so that the paraffin is not fully immersed in the tissue, resulting in tissue deformation, section failure, etc., so the whole paraffin section production process needs a lot of time to explore the processing time, reagent conditions, processing methods, etc.

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