Biochemical reagent and preparation method and application thereof
A technology of biological reagents and equipment, applied in the unique preparation of biological reagents, in the field of biological reagents, can solve the problems of biological repair technology that is difficult to promote, does not have large-scale pollution control, low cost, and has no impact on the environment, and achieves low mixing effect, Low cost and the effect of increasing the concentration of microorganisms
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Embodiment 1
[0025] Biological reagent is prepared by mixed spray reaction of solid phase spray and liquid phase spray; the mass ratio of solid phase spray and liquid phase spray is 1:0.4; the solid phase spray is; the liquid phase spray includes the following parts by weight Components: 5 parts of composite bacteria, 15 parts of nutrient, and 0.05 part of binder; wherein, the composite bacteria are Bacillus subtilis CMCC 63501 and photosynthetic bacteria Rhodopseudomonas palustris DSM5859 with a mass ratio of 1:4, purchased from In Jiangsu Lvke Biotechnology Co., Ltd.; the nutritional agent includes the following components in parts by weight: 0.07 parts of yeast extract, 0.03 parts of beef extract, 0.1 part of peptone, 0.1 part of glucose, 0.05 parts of sodium chloride, 0.02 parts of ammonium sulfate, 0.02 part of magnesium sulfate heptahydrate, 0.1 part of dipotassium hydrogen phosphate.
[0026] The preparation steps of this biological reagent are as follows:
[0027] (1) Prepare the ...
Embodiment 2
[0031] The biological reagent is prepared by the mixed spray reaction of solid phase spray and liquid phase spray; the mass ratio of solid phase spray and liquid phase spray is 1:0.5; the solid phase spray is; the liquid phase spray includes the following parts by weight Components: 5 parts of composite bacteria, 15 parts of nutrient, and 0.15 parts of binder; wherein, the composite bacteria are Bacillus subtilis and photosynthetic bacteria Rhodospiraceae red non-sulfur bacteria with a mass ratio of 1:6; The nutritional agent includes the following components by weight: 0.05 parts of yeast extract, 0.06 parts of beef extract, 0.15 parts of peptone, 0.15 parts of glucose, 0.02 parts of sodium chloride, 0.05 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, dipotassium hydrogen phosphate 0.05 parts.
Embodiment 3
[0033] Biological reagent is prepared by mixed spray reaction of solid phase spray and liquid phase spray; the mass ratio of solid phase spray and liquid phase spray is 1:0.6; the solid phase spray is; the liquid phase spray includes the following parts by weight Components: 15 parts of composite bacteria, 5 parts of nutrient, and 0.05 parts of binder; wherein, the composite bacteria are Bacillus subtilis and photosynthetic bacteria Rhodospiraceae red non-sulfur bacteria with a mass ratio of 1:4; The nutritional agent includes the following components by weight: 0.05 parts of yeast extract, 0.06 parts of beef extract, 0.15 parts of peptone, 0.15 parts of glucose, 0.02 parts of sodium chloride, 0.05 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, dipotassium hydrogen phosphate 0.05 parts.
[0034] The biological reagent prepared in Example 1 was used for wastewater treatment. The polluted sediment and polluted water used in the experiment come from a s...
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