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Biochemical reagent and preparation method and application thereof

A technology of biological reagents and equipment, applied in the unique preparation of biological reagents, in the field of biological reagents, can solve the problems of biological repair technology that is difficult to promote, does not have large-scale pollution control, low cost, and has no impact on the environment, and achieves low mixing effect, Low cost and the effect of increasing the concentration of microorganisms

Inactive Publication Date: 2015-08-19
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method often treats the symptoms but not the root cause, and the aeration and reoxygenation technology and the sediment dredging technology all have the disadvantage of large investment, and do not have the ability to control pollution on a large scale, and the final treatment of the polluted sediment removed in the sediment dredging is also a problem. It is a difficult problem; the chemical method mainly uses various chemical agents, such as adding chemical agents to kill algae, adding iron salts, etc. to promote the precipitation of phosphorus (chemical phosphorus fixation), etc. Although chemical methods can temporarily improve water quality, they will cause Secondary pollution; Biological method is a main body repair technology with good development prospects. It has low treatment cost, no impact on the environment, and can degrade pollution to the maximum extent. Difficult to promote technology

Method used

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  • Biochemical reagent and preparation method and application thereof
  • Biochemical reagent and preparation method and application thereof
  • Biochemical reagent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Biological reagent is prepared by mixed spray reaction of solid phase spray and liquid phase spray; the mass ratio of solid phase spray and liquid phase spray is 1:0.4; the solid phase spray is; the liquid phase spray includes the following parts by weight Components: 5 parts of composite bacteria, 15 parts of nutrient, and 0.05 part of binder; wherein, the composite bacteria are Bacillus subtilis CMCC 63501 and photosynthetic bacteria Rhodopseudomonas palustris DSM5859 with a mass ratio of 1:4, purchased from In Jiangsu Lvke Biotechnology Co., Ltd.; the nutritional agent includes the following components in parts by weight: 0.07 parts of yeast extract, 0.03 parts of beef extract, 0.1 part of peptone, 0.1 part of glucose, 0.05 parts of sodium chloride, 0.02 parts of ammonium sulfate, 0.02 part of magnesium sulfate heptahydrate, 0.1 part of dipotassium hydrogen phosphate.

[0026] The preparation steps of this biological reagent are as follows:

[0027] (1) Prepare the ...

Embodiment 2

[0031] The biological reagent is prepared by the mixed spray reaction of solid phase spray and liquid phase spray; the mass ratio of solid phase spray and liquid phase spray is 1:0.5; the solid phase spray is; the liquid phase spray includes the following parts by weight Components: 5 parts of composite bacteria, 15 parts of nutrient, and 0.15 parts of binder; wherein, the composite bacteria are Bacillus subtilis and photosynthetic bacteria Rhodospiraceae red non-sulfur bacteria with a mass ratio of 1:6; The nutritional agent includes the following components by weight: 0.05 parts of yeast extract, 0.06 parts of beef extract, 0.15 parts of peptone, 0.15 parts of glucose, 0.02 parts of sodium chloride, 0.05 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, dipotassium hydrogen phosphate 0.05 parts.

Embodiment 3

[0033] Biological reagent is prepared by mixed spray reaction of solid phase spray and liquid phase spray; the mass ratio of solid phase spray and liquid phase spray is 1:0.6; the solid phase spray is; the liquid phase spray includes the following parts by weight Components: 15 parts of composite bacteria, 5 parts of nutrient, and 0.05 parts of binder; wherein, the composite bacteria are Bacillus subtilis and photosynthetic bacteria Rhodospiraceae red non-sulfur bacteria with a mass ratio of 1:4; The nutritional agent includes the following components by weight: 0.05 parts of yeast extract, 0.06 parts of beef extract, 0.15 parts of peptone, 0.15 parts of glucose, 0.02 parts of sodium chloride, 0.05 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, dipotassium hydrogen phosphate 0.05 parts.

[0034] The biological reagent prepared in Example 1 was used for wastewater treatment. The polluted sediment and polluted water used in the experiment come from a s...

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Abstract

The invention provides a biochemical reagent, which is prepared from solid phase spray and liquid phase spray by mixing spray reaction; the mass ratio of the solid phase spray to the liquid phase spray is 1:0.4-0.6; the solid phase spray is composite non-metallic minerals A (patent number 201310017703.0); the liquid phase spray comprises the following components in parts by weight: 5-15 parts of compound bacteria, 5-15 parts of nutritional agent and 0.05-0.15 parts of binder, wherein the compound bacteria are bacillus subtilis and photosynthetic bacteria of which the mass ratio is 1:4-6. The invention provides mixing spray reaction equipment used for preparing the biochemical reagent and a method for preparing the biochemical reagent by using the equipment. The biochemical reagent prepared according to the invention takes into account two aspects of microorganism loading capacity and substrate mass transfer resistance, low cost and long service life are realized, and thus, the biochemical reagent and the preparation method and application thereof can be used for treating polluted water bodies of a city and used for various water treatment engineering projects, and have a bright prospect.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a biological reagent, and also relates to a unique preparation method and application of the biological reagent. Background technique [0002] At present, water pollution has become one of the most important problems of urban environment in our country. Rivers and lakes across the country are generally polluted to varying degrees, especially in the middle and lower reaches of large and medium-sized cities. Urban rivers are an important part of my country's water environment. Due to the discharge of industrial wastewater and urban domestic sewage, urban rivers are seriously polluted and eutrophication is common. According to statistics, by the beginning of the 20th century, there was almost no complete natural river in the world, and the severe damage and degradation of urban rivers has been recognized as a global ecological environment problem. It can be seen that the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12M1/00C12M1/04C12M1/02C02F3/34C12R1/125
Inventor 祝建中张晖季敏吉栋梁
Owner HOHAI UNIV
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