A molecular identification method for rice black-streaked dwarf resistance

A rice black-streaked dwarf and molecular identification technology, which is applied in the molecular identification of rice black-streaked dwarf resistance and the field of molecular identification of rice black-streaked dwarf resistance, can solve the problem of short identification period and long identification period , low accuracy and other issues

Active Publication Date: 2018-10-09
JIANGSU FOCUS AGRICULTURE SCIENCE AND TECHNOLOGY CO. LTD.
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a rice black-streaked dwarf disease with a short identification period and high accuracy in view of the shortcomings of the existing rice black-streaked dwarf variety resistance identification method that has a long identification period and low accuracy. Molecular identification method of cultivar resistance

Method used

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  • A molecular identification method for rice black-streaked dwarf resistance
  • A molecular identification method for rice black-streaked dwarf resistance
  • A molecular identification method for rice black-streaked dwarf resistance

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Experimental program
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Embodiment

[0045] In 2015, using the identification method of the invention, the black-streaked dwarf disease resistance identification was carried out on some of the collected rice variety resources. The specific steps are as follows:

[0046] 1) Acquisition of poisonous SBPH and identification scale: Collect senior SBPH nymphs in the field, purify, screen and preserve them for passage. Before identification, use RBSDV poison source to feed 2-instar SBPH nymphs to obtain poisonous SBPH; according to In the artificial inoculation identification, three identification scales are set for the resistance performance of different varieties to rice black-streaked dwarf disease: high resistance, medium resistance, and susceptibility. The specific operation steps are as follows:

[0047] 1.1) Acquisition of RBSDV virus source: Collect diseased rice plants showing symptoms of rice black-streaked dwarf disease at the peak tillering stage from the field in the severely affected area of ​​rice black-s...

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Abstract

The invention discloses a molecular identification method for the resistance of a rice black streaked dwarf virus (RBSDV). The identification method comprises the following steps of: (1) obtaining brown planthoppers with the virus and an identification ruler; (2) performing quantitative virus transmission to coleoptiles on the brown planthoppers with the virus; (3) performing fluorescent quantitative polymerase chain reaction (PCR) to obtain the relative quantity (RQ) value of each variety cycle time point; and (4) drawing an RBSDV proliferation index trend line and performing resistance evaluation. The molecular identification method has the advantages that the resistance level and resistance grade of rice varieties for the rice black streaked dwarf virus can be accurately identified on the molecular level at the rice sprouting period, the identification period is short, the accuracy is high, and thus the method has relatively high breeding application values.

Description

technical field [0001] The invention belongs to the field of biological and current agricultural high-tech, and relates to a molecular identification method for rice black-streaked dwarf resistance, in particular, to a rice black-streaked dwarf resistant variety with short identification period and high accuracy. Molecular identification methods. Background technique [0002] Rice black-streaked dwarf virus (RBSDV) is caused by rice black-streaked dwarf virus (RBSDV). Laodelphax striatellus Fallen), which is widely distributed in East Asia such as China, North Korea, South Korea and Japan. The main symptoms of rice black-streaked dwarf disease are dwarf plants, dark green leaves, increased tillers, shortened and stiff leaves, waxy white veins on the back of leaves, leaf sheaths and stem surfaces, and in the later stage of the disease, it becomes longitudinal along the veins. Symptoms such as dark brown tumor-like protrusions, small ears or no heading, and poor firmness, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12Q1/686C12R1/93
CPCC12Q1/6851C12Q1/686C12Q1/70C12Q2531/113C12Q2545/101C12Q2561/101
Inventor 不公告发明人
Owner JIANGSU FOCUS AGRICULTURE SCIENCE AND TECHNOLOGY CO. LTD.
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