A leptin active peptide with a mutation in the c-helix region, its coding gene and application

Abstract

The invention discloses a leptin active peptide with a mutation in the C-helix region, its coding gene and application. The amino acid sequence of the leptin active peptide mutated in the C helical region is shown in SEQ ID NO.1. The invention modifies the amino acid sequence of human Leptin to improve its fat degradation efficiency. Using chemical methods to synthesize leptin active peptides with mutations in the C-helix region, as much as possible to reduce the impact of protein space structure on its activity, thereby improving the targeting and degradation of fat cells, the actual results show that the C-helix of the present invention The adipocyte degradation activity of the leptin active peptide mutated in the region was significantly better than that of the positive control-human leptin protein and the commercially available drug rosiglitazone. Obvious cell lysis effect, and the effect is significantly better than H-LEP and rosiglitazone, thus providing a candidate target for the development of new, efficient and cheap weight loss and hypoglycemic drugs.

Description

Technical field: [0001] The invention belongs to the fields of biochemistry and molecular biology, and in particular relates to a leptin active peptide with a mutation in the C-helix region, its coding gene and its application. Background technique: [0002] Obesity is one of the greatest enemies of human health. It is not only an independent disease, but also a cause of type 2 diabetes, cardiovascular disease, hypertension, stroke and various cancers. It is listed by the World Health Organization as the cause of disease burden. One of the top ten threats is a global problem that cannot be ignored. According to reports, about 18 million people die from cardiovascular and cerebrovascular diseases caused by diabetes and hypertension every year in the world, and obesity is a key pathogenic factor of diabetes and hypertension. Obesity has seriously endangered human health and caused huge losses to society. According to reports, in 2003, my country's expenditure on obesity, dia...

AI Technical Summary

Problems solved by technology

Subsequently, American scientists took the lead in conducting the first human leptin protein experiment. However, among the 73 obese patients tested, most of the patients had no obvious weight loss effect, and only 2 patients lost 35 pounds within 24 weeks, which shows that It is difficult to obtain ideal weight loss effect with wild-type human leptin protein

Images