Artificial inoculation method of alternaria alternata

Abstract

The invention discloses an artificial inoculation method of alternaria alternata, in which an alternaria alternata spore suspension liquid is injected through wounds on in-vitro leaves to performing inoculation to manure leaves, wherein conidium of the alternaria alternate can directly enter the leaves so that the conidium can be germinated and reproduced with moisture and host mesophyll tissue nutrition and then is extended intercellularly, so that disease spots formed by local tissue death is enlarged in a large area, thereby achieving effects of high inoculation rate and moderate disease degree. The invention provides the simple, convenient, quick and accurate artificial inoculation method for identification of pathogenicity and interspecific difference of different alternaria alternata in main production area of tobacco in our country and screening of resistant variety of the alternaria alternata. The artificial inoculation method has an excellent application value.

Description

technical field [0001] The invention relates to a method for inoculating pathogens in the field of plant protection, in particular to an artificial inoculation method for tobacco red spot disease. Background technique [0002] Tobacco red spot is a leaf fungal disease caused by Alternaria alternata Fries Keissler, which occurs in the middle and late stages of tobacco growth and is one of the most threatening diseases in tobacco production. The disease has the characteristics of short incubation period and rapid epidemic speed. Under suitable environmental conditions, it can cause a large epidemic in a short period of time and bring huge losses to tobacco leaf production. Since the 1990s, the incidence of tobacco red spot disease in my country has increased year by year, and it has become more and more serious, becoming one of the main infectious diseases. Therefore, the scientific control of red spot disease is an urgent problem to be solved in production. The use of disea...

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Problems solved by technology

At present, the reported artificial inoculation methods of Alternaria Alternaria mainly include mycelium block inoculation method (Dong Hansong, Qu Jianjun, Lu Shien, etc. Research on Spore Germination and Infection Conditions of Tobacco Alternaria Alternaria Shandong Science 1993 (2): 30-35), Mycelia hanging drop inoculation method (Shen Yi, Gao Zhimou, Du Lei et al. Research on artificial inoculation method of tobacco red spot fungus seedling stage Anhui Agricultural University Journal 2009,36(3):356-359), spore hanging drop method (Zhang Shicai. Pathogenicity differentiation and genetic difference of tobacco red spot disease in Chongqing. 2009, master's degree dissertation of Southwest University), spore liquid cotton ball method (Cheng Julong, Sun Wei. Resistance performance and synthesis of tobacco red spot disease at different developmental stages Control technology. Chinese Journal of Plant Protection 2001,28(1):44-48) and spraying method (Jiao Tianlei. Mapping analysis of QTL for resistance to red spot disease in tobacco (Nicotiana tabacum L.). 2011, Master's degree thesis of Zhejiang University); but in In the experiment, it was found that when using the above-mentioned several different methods to inoculate the disease of red spot disease, the impact on the incidence of red spot disease was very different, and there was a problem that the inoculation did not cause the disease or the disease was not ideal, and the immaturity of the inoculation method would limit the The development of research work on the interaction between pathogenic bacteria and host

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