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Chromatographic method for determining folic acid content in health food

A chromatographic method and technology for health food, which are applied in the field of chromatography for determining the content of folic acid in health food to achieve the effect of accurate determination

Inactive Publication Date: 2015-07-29
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the determination results of folic acid in some particularly complex component samples are far from the theoretical value, and it is necessary to establish a more accurate and reliable method based on this

Method used

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  • Chromatographic method for determining folic acid content in health food
  • Chromatographic method for determining folic acid content in health food

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with specific embodiments.

[0031] Such as figure 1 As shown, the determination of folic acid content in One Centure active adults 50+ samples:

[0032] (1) Test sample processing: Take 20 pieces of One Centure active adults 50+ samples and grind them finely, accurately weigh the samples equivalent to 1 mg of folic acid, put them in a 50 ml brown volumetric flask, add 0.3 g of DTPA and 0.5 g of vitamin C, and use 0.5% ammonia water is ultrasonically dissolved, and the pH is controlled at 8~9, and then a small amount of 0.5% ammonia water is used to make up to volume; take an appropriate amount of the obtained solution in a centrifuge tube, centrifuge for about 15 min, and take the supernatant and filter it with a 0.45um filter membrane before entering Sample.

[0033] (2) Preparation of reference substance solution: Accurately weigh 20 mg of folic acid reference substance into a 100 ml brown volume...

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Abstract

The present invention discloses a chromatographic method for determining the folic acid content in a health food. The chromatographic method comprises: (1) detection sample treatment; (2) standard substance preparation; (3) chromatographic condition setting; and (4) sample control, wherein diethylenetriamine pentaacetic acid (DTPA) and vitamin C are added during the detection sample treatment process so as to adjust the ion strength and mask the interference of the trace element ions on the detection, such that the direct and accurate determination of the folic acid content in the health product is achieved. According to the present invention, the chromatographic detection result of the technology is accurate and reliable, the problem that the result of the traditional determination process deviates from the actual value is solved, and the actual determination requirements are met.

Description

technical field [0001] The invention belongs to the specific application of high performance liquid chromatography (HPLC) in the analysis of food and drug components. Specifically, through the ingenious selection of chromatographic conditions, the accurate qualitative and quantitative analysis of the content of folic acid in the actual sample of specific complex components is realized. analyze. Background technique [0002] Folic acid is an important B vitamin, its chemical name is Dicotyloglutamic Acid, which has the effects of promoting lactation, building skin, preventing gray hair, increasing appetite and preventing oral ulcers. It is naturally present in green leafy vegetables and animal meat and eggs. In milk and liver, the content in the liver is the most abundant. In addition to natural existence, folic acid is added to some nutritionally fortified foods and health foods as an additive in an appropriate amount. Accurate determination of folic acid content is of grea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 盛洁包亚君
Owner JIANGSU ALAND NOURISHMENT
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