Preparation method of pleurotus citrinopileatus amino acid powder
A technology of amino acid powder and yellow mushroom, applied in fermentation and other directions, can solve the problems of not fully utilizing the nutritional value of yellow mushroom and less utilization of amino acid components, and achieve the effect of good embedding effect, high purity and high yield
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Embodiment 1
[0025] A preparation method of Pleurotus ostreatus amino acid powder includes the following steps:
[0026] (1) Washing and pre-cooking: Wash the hummus mushrooms in pure water to remove impurities such as sediment, and then cut them into 1 to 3 cm sections, pre-cook them in water at 90°C for 5 minutes, and remove them.
[0027] (2) Beating: beat the pure water 8 times the weight of the Pleurotus citrinopileatus with the small sections of the Pleurotus citrinopileatus obtained in step (1), and then perform ultrasonic-assisted crushing under the condition of a frequency of 60-100 Hz for 8 min.
[0028] (3) Enzymatic hydrolysis: using endopeptidase and terminal peptidase to enzymatically hydrolyze the by-product pulp of Pleurotus ostreatus. First adjust the pH to 4.0 and the temperature to 45°C, add endopeptidase for enzymatic hydrolysis, and the enzymatic hydrolysis time is 3h; then adjust the pH to 5.0 and the temperature to 15°C, add telopeptase for enzymatic hydrolysis, and the enz...
Embodiment 2
[0034] A preparation method of Pleurotus ostreatus amino acid powder includes the following steps:
[0035] (1) Washing and pre-cooking: Wash the Pleurotus citrinopileatus in clean water to remove impurities such as sediment, and then cut it into small sections of 1 to 3 cm, pre-cook them in 95°C water for 6 minutes, and remove them.
[0036] (2) Beating: beat the pure water 12 times the weight of the Pleurotus citrinopileatus with the small sections of Pleurotus citrinopileatus obtained in step (1), and then perform ultrasonic-assisted crushing under the condition of a frequency of 60-100 Hz for 10 min.
[0037] (3) Enzymatic hydrolysis with complex enzymes: Endopeptidase and telopeptidase are used to hydrolyze the by-product pulp of Pleurotus ostreatus. First adjust the pH to 4.5 and the temperature to 50°C, add endopeptidase for enzymolysis, and the enzymolysis time is 4h; then adjust the pH to 5.5, the temperature to 30°C, add telopeptase for enzymolysis, and the enzymolysis time...
Embodiment 3
[0043] A preparation method of Pleurotus ostreatus amino acid powder includes the following steps:
[0044] (1) Washing and pre-cooking: Wash the hummus mushrooms in pure to remove impurities such as sediment, and then cut them into small sections of 1 to 3 cm, pre-cook them in 95°C water for 8 minutes, and remove them.
[0045] (2) Beating: beat the pure water 15 times the weight of the Pleurotus citrinopileatus with the small sections of Pleurotus citrinopileatus obtained in step (1), and then perform ultrasonic-assisted crushing under the condition of a frequency of 60-100 Hz for 12 min.
[0046] (3) Enzymatic hydrolysis with complex enzymes: Endopeptidase and telopeptidase are used to hydrolyze the by-product pulp of Pleurotus ostreatus. First adjust the pH to 4.5 and the temperature to 55°C, add endopeptidase for enzymolysis, the enzymolysis time is 5h; then adjust the pH to 5.5, the temperature to 55°C, add telopeptase for enzymolysis, the enzymolysis time is 7h.
[0047] (4) Fi...
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