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Application of Chinese medicine monomer (lycorine) to preparation of drugs for treating prostate tumors

A technology of lycorine and prostate cancer, applied in the field of medicine, can solve the problems of unsatisfactory curative effect of prostate cancer, recurrence of multidrug-resistant tumors, poor targeting of anti-prostate cancer drugs, etc.

Inactive Publication Date: 2015-07-29
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, despite the continuous emergence of new chemotherapy drugs and continuous improvement of chemotherapy regimens in recent years, anti-prostate cancer drugs still generally have problems such as poor drug targeting and strong side effects, which limit the efficacy and long-term use of drugs. Multidrug resistance has been the main reason for tumor recurrence, metastasis and even treatment failure, which is also the most common and most difficult problem in clinical prostate cancer treatment
The curative effect of commonly used chemotherapeutic drugs on prostate cancer is not very satisfactory, which makes finding active ingredients from natural substances an important development strategy for the development of anti-prostate cancer drugs

Method used

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  • Application of Chinese medicine monomer (lycorine) to preparation of drugs for treating prostate tumors
  • Application of Chinese medicine monomer (lycorine) to preparation of drugs for treating prostate tumors
  • Application of Chinese medicine monomer (lycorine) to preparation of drugs for treating prostate tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Lycorine inhibits the proliferation, migration and invasion of prostate cancer cells

[0027] Technical method

[0028] 1. Cell culture and cell survival experiments

[0029] Prostate cancer cells PC-3M, LNCaP, 22RV1 and DU145 used in the present invention were purchased from ATCC cell bank, and human normal prostate epithelial cell line PNT1A was obtained from Shanghai Jiaotong University. The cells were cultured in a constant temperature incubator at 37°C (humidity 95%, CO 2 Concentration 5%), the medium is RPMI1640 containing 10% fetal bovine serum. Cell viability was determined by MTS method. Prostate cell lines PC-3M, LNCaP, 22RV1 and DU145, and human normal prostate epithelial cell line PNT1A in 5x10 3 Cells / well density was inoculated into 96-well plates, and different concentrations of the monomer compound were added after 24 hours, and the same amount of DMSO was added to the control group, and 3 replicate wells were set up in each group. After ...

Embodiment 2

[0036] Example 2: Lycorine inhibits the growth of prostate cancer cells in vitro by inducing apoptosis

[0037] Technical method

[0038] 1. Colony formation experiment

[0039] Digest and process the corresponding cells in the logarithmic growth phase, and count, 1x10 per well 3 Cells were seeded in a six-well plate to ensure that the seeded cells were evenly distributed. After the cells adhere to the wall, change the medium and add the complete medium containing the corresponding concentration of the monomer compound. After culturing for one week, suck up the original culture medium with a suction pump, wash 3 times with phosphate buffer solution, fix the cells with paraformaldehyde solution (20 min), then stain the cells with 2‰ crystal violet staining solution for 5 min, and finally use Rinse gently with slow-flowing tap water to wash away unbound crystal violet staining solution, and dry naturally at room temperature. Photographs were taken under a microscope, and the...

Embodiment 3

[0048] Example 3: Inhibitory effect of lycorine on subcutaneous tumor-bearing growth of mouse prostate cancer model

[0049] Technical method

[0050] Will 1×10 6 Personal prostate cancer cell PC-3M was subcutaneously injected into the back of immunodeficient mice (BLAB / c-nude, nude mice) until the subcutaneous tumor grew to 100mm 3 When left and right, the mice were divided into three groups (10 mice in each group). The mice in the low-dose group were injected intraperitoneally with 5 mg / kg lycorine dissolved in DMSO every day, the mice in the high-dose group were injected intraperitoneally with 10 mg / kg lycorine dissolved in DMSO every day, and the mice in the control group were injected with the same volume of DMSO. The body weight of the mice and the length and width of the tumor were recorded, and the mice were sacrificed after 18 days of continuous drug administration. Subcutaneous tumors were removed and photographed. According to the formula volume = length x width...

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Abstract

The invention discloses application of a Chinese herbal medicine micro-molecule monomer compound (lycorine) to preparation of potential drugs for treating prostate tumors and related diseases. The invention further discloses a prostate cancer resistance molecular mechanism of the Chinese herbal medicine micro-molecule monomer compound.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Chinese herbal medicine monomer lycorine in the preparation of a potential drug for treating prostate malignant tumors. Background technique [0002] Worldwide, prostate cancer is the number one killer of malignant tumors in elderly men worldwide, and its morbidity and mortality rate ranks at the forefront of all cancer types in western developed countries; in my country, prostate cancer has also increased by 10% per year in recent years. The speed of the disease is increasing rapidly, especially as my country is entering an aging society, and the incidence peak is likely to appear in the next ten years. Due to its anatomical concealment, once diagnosed, most prostate cancers are already in the middle and advanced stages, thus losing the chance of radical treatment. Early prostate cancer is generally treated radically through surgery such as removal of the tes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4745A61P35/00
Inventor 易正芳胡美纯彭世鸿邢雅婧刘明耀
Owner EAST CHINA NORMAL UNIV
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