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Molecular marker wgrb125 of wheat flag leaf width gene taflw1 and its application

A molecular marker and wheat technology, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of few research reports on the genetic location of flag leaf size, so as to improve the efficiency of breeding selection and solve the linkage burden , the effect of convenient detection

Active Publication Date: 2017-07-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies on the correlation between flag leaf traits and yield traits and the mapping of yield traits QTL have also been carried out earlier in wheat, but there are few reports on the genetic mapping of flag leaf size

Method used

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  • Molecular marker wgrb125 of wheat flag leaf width gene taflw1 and its application
  • Molecular marker wgrb125 of wheat flag leaf width gene taflw1 and its application
  • Molecular marker wgrb125 of wheat flag leaf width gene taflw1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Obtainment of Co-segregation Molecular Marker of Wheat Flag Leaf Wide Gene TaFLW1

[0043] (1) Secondary F derived from Wangshuibai Taflw1 near isogenic line (NIL) 2 Population Construction and Screening of TaFLW1 Segment Recombinants

[0044] (1) NIL(♀) was crossed with its recurrent parent PH691(♂) to obtain the hybrid F 1 , F 1 After selfing produced F containing 4661 individuals 2 group;

[0045] (2) Extract F by SDS method 2 DNA from each individual plant in the population; obtained from F 2 Eight heterozygous individuals recombined in this segment were screened in the population, and one homozygous recombined individual was screened in each of its selfed offspring using the same marker.

[0046] (2) Identification of 8 homozygous recombinants with wide flag leaf phenotype

[0047] The seeds harvested after selfing of 8 homozygous recombinant single plants were used to identify the flag leaf width phenotype in the field; the identification was carr...

Embodiment 2

[0055] Example 2 Application of Wheat Flag Leaf Width Gene TaFLW1 Co-segregation Molecular Marker

[0056] The flag leaf width phenotype was identified on a wheat micro-core collection containing 262 accessions. The identification was carried out in Fengyang Experimental Field, Anhui in 2013, and the identification method was the same as in Example 1. Genomic DNA of 262 materials was extracted by SDS method and PCR amplified by WGRB125. The PCR amplification method is the same as in Example 1. The amplified products were separated by electrophoresis on 8% non-denaturing polyacrylamide gel, then silver-stained and photographed, and the results were recorded.

[0057] The results showed that among the 262 wheat micro-core germplasm materials, 40 materials could amplify 186bp amplified fragments, and the average flag leaf width was 20.0mm; 119 materials could amplify 188bp amplified fragments , with an average flag leaf width of 17.9 mm. The average flag leaf width of materia...

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Abstract

The invention belongs to the field of crop breeding, and discloses a molecular marker WGRB125 of a wheat flag leaf wide gene TaFLW1 and application thereof. The molecular marker WGRB125 and TaFLW1 gene have genetic distance of 0cM. The invention obtains the molecular marker in closest linkage with TaFLW1 for the first time internationally. WGRB125 is a codominant marker and has the advantages of convenience for detection, amplification stability and simpleness. The marker WGRB125 for detecting TaFLW1 gene can the determine the existence and existing state of TaFLW1 and predict the width of flag leaf of wheat, so as to rapidly screen the plants with TaFLW1 and apply to the breeding of high yield varieties.

Description

technical field [0001] The invention belongs to the field of crop breeding and relates to a molecular marker of wheat flag leaf width gene TaFLW1 and its application. [0002] technical background [0003] Wheat is one of the most important food crops in the world, and its production is indispensable for human food security. Wheat yield is a complex trait, not only directly affected by the three factors of yield, but also closely related to other aspects, such as source-sink relationship (Cui et al.2003). The leaf, as the main organ of the "source", has an important influence on the yield potential of wheat. Among them, the photosynthesis of the topmost flag leaf accounts for 45–58% of the photosynthetic output of all leaves. During the grain filling stage, the carbohydrates produced by the photosynthesis of the flag leaves contributed 41–43% to the dry matter accumulation of the grain, and were the most important source of assimilates to the ear, which played a key role in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马正强薛树林秦琦
Owner NANJING AGRICULTURAL UNIVERSITY
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