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Method for preparing biological nanoselenium by using Rahnella aquatilis

An aquatic Lahnella and bio-nano technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve good economic and social benefits, great application potential, and less impurities

Active Publication Date: 2015-07-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, Rahnella aquatilis HX2 has a good effect of dissolving phosphorus, produces plant biomass-promoting substances IAA and PQQ, and can produce plant antibacterial substances at the same time, which has a disease prevention and growth-promoting effect on plants. A report on the synthesis of bionano-selenium by Lahnella

Method used

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  • Method for preparing biological nanoselenium by using Rahnella aquatilis
  • Method for preparing biological nanoselenium by using Rahnella aquatilis
  • Method for preparing biological nanoselenium by using Rahnella aquatilis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Lahnella aquatica is to sodium selenite (Na 2 SeO 3 ) tolerance test

[0033] 1. LB liquid medium: NaCl 10g, tryptone 10g, yeast extract 5g, deionized water 1L, autoclaved at 121°C for 20min.

[0034] LB solid medium: NaCl 10g, tryptone 10g, yeast extract 5g, agar powder 15g, deionized water 1L, aliquoted into 100mL Erlenmeyer flasks (25mL / bottle), autoclaved at 121°C for 20min.

[0035] Add the 1M sodium selenite mother liquor that filters and sterilizes in advance respectively in the LB solid culture medium after above-mentioned autoclaving and be mixed with following concentration 0mM (CK), 60mM, 70mM, 75mM, 85mM, 95mM, 105mM, 115mM containing LB medium with sodium selenite.

[0036] 2. Draw 100 μL of the activated Lahnella aquatica HX2 bacterial solution (OD 600 =0.8), diluted with 0.85% normal saline, and the final dilution was 10 -3 、10 -4 、10 -5 、10 -6 The bacterial solution is ready for use.

[0037] 3. Pipette 2.5 μL of the above gradient ...

Embodiment 2

[0040] Embodiment 2 Lahnella aquatica to sodium selenate (Na 2 SeO 4 ) tolerance test

[0041] 1. Prepare LB sterile solid medium plates containing sodium selenate concentrations of 0mM (CK), 475mM, 500mM, 525mM, 550mM, 575mM, 600mM, and 625mM respectively. (wherein the LB solid culture medium compounding method is with embodiment 1, adds respectively the sodium selenate mother liquor of the 3M of filter sterilization in advance and is mixed with the LB culture medium of corresponding concentration selenium content)

[0042] 2. Take 100 μL of the activated Lahnella aquatica HX2 bacterial solution (OD 600 =0.8) was gradually diluted with 0.85% normal saline, and the final dilution was 10 -3 、10 -4 、10 -5 、10 -6 The bacterial solution is ready for use.

[0043] 3. Aspirate 2.5uL of the above gradient (10 -3 、10 -4 、10 -5 、10 -6 ) was added dropwise to LB plates containing different selenium concentrations, and each gradient was repeated three times. After the bacteria...

Embodiment 3

[0046] Embodiment 3 uses Lahnella aquatica HX2 to use sodium selenite (Na 2 SeO 3 ) as raw material to biosynthesize nano-selenium

[0047] After activating Lahnella aquatica HX2, transfer 1% bacterial solution (OD 600 =0.8) In the sterilized Erlenmeyer flask containing LB liquid medium, set up 3 test groups, add a certain volume of 1M Na 2 SeO 3 Mother liquor, to ensure Na in the Erlenmeyer flask in each test group 2 SeO 3 The final concentrations were 5, 15, 60, and 75 mM, respectively, placed in a shaker (28° C., 180 rpm), and the corresponding incubation times were 96, 88, 80, and 72 hours.

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Abstract

The invention provides a method for preparing biological nanoselenium by using Rahnella aquatilis, and belongs to the fields of microbe application and nanoselenium synthesis. The method is characterized culturing is carried out with selenate or selenite as a raw material and Rahnella aquatilis as a zymocyte, and a fermentation culturing solution is collected, separated and purified to obtain the nanoselenium. The method has the advantages of cleanness, non-toxicity, environmental protection and high efficiency, and the synthesized nanoselenium has the advantages of stable properties, few impurities, uniform particle size and good dispersion, and can be used to produce selenium-rich products. The method makes up the disadvantages of traditional methods for producing nanoselenium and the selenium-rich products to realize environmental protection and high efficiency effects, can be widely applied to fields of selenium-rich product exploitation and the like, and has good economic and social benefits.

Description

technical field [0001] The invention relates to the field of microbial application and nano-selenium synthesis, in particular to a method for preparing biological nano-selenium by using Lahnella aquatica. Background technique [0002] Selenium (Se) is an essential trace element for animals and humans, and is inseparable from the antioxidant function in the human body, thyroid hormone metabolism, and the immune system. Selenium deficiency can cause human muscle atrophy, cardiovascular, skeletal or immune system diseases, and increase the risk of cancer, but excessive selenium can also be toxic to the human body. However, the distribution of selenium in my country is extremely uneven, and selenium-deficient regions occupy a considerable proportion, which urgently needs to be solved. At present, selenium-enriched products by supplementing plants are the most widely used, and their production mainly relies on the application of selenium fertilizers to supplement selenium salts,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 郭岩彬吴文良李吉祥朱燕云任宝玉赵桂慎
Owner CHINA AGRI UNIV
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