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Organic additive induced goodyera foliosa plant regeneration and efficient-propagation method

A multi-leaf variegated orchid and organic additive technology, which is applied in the directions of plant regeneration, botanical equipment and methods, horticultural methods, etc. The effect of shortening the process of seedling cultivation, high technical content and strong public welfare

Inactive Publication Date: 2015-07-15
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the plant regeneration and rapid propagation technology of variegated orchid

Method used

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  • Organic additive induced goodyera foliosa plant regeneration and efficient-propagation method
  • Organic additive induced goodyera foliosa plant regeneration and efficient-propagation method
  • Organic additive induced goodyera foliosa plant regeneration and efficient-propagation method

Examples

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Embodiment 1

[0027] A method for inducing plant regeneration and rapid propagation of variegated orchid, the specific steps are as follows:

[0028] 1) Material selection and disinfection: Rinse the collected wild plants with running water, cut off the leaves on the plants, soak in saturated bleach supernatant for 10 minutes, and scrub with a soft brush. After rinsing, rinse under tap water for 20 minutes , then rinsed twice with double distilled water, placed in an ultra-clean workbench, sterilized with 75% alcohol for 30 seconds, added 0.1% mercuric chloride for 10 minutes, rinsed three times with sterile water, blotted the surface moisture with sterile filter paper, and inoculated ;

[0029] 2) Induction culture: cut the sterilized materials into 1 cm long stems with 1 node and inoculate them in the bud induction medium sterilized by high temperature and high pressure; culture conditions: the temperature of the culture room is 23°C , light time 10h / d, light intensity 1800lx, culture ti...

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Abstract

The invention belongs to rapid propagation methods for important plant materials in endangered wild plants and particularly relates to tissue culture methods for wild orchids. The invention aims at providing an organic additive induced goodyera foliosa plant regeneration and efficient-propagation method which comprises the steps of selecting and disinfecting a material, carrying out induced culture, carrying out propagation culture, carrying out root induction and transplanting, wherein a bud induction culture medium, a propagation culture medium and a rooting culture medium are involved. According to the formulae of culture media, the coefficient of propagation is large, the rooting rate is high, the plant growth required time is short, after cultivating, the adaptability is strong, and nursery-grown plants are robust, tall and straight and good, orderly and consistent in growth vigor, so that a nursery-grown plant culture process is greatly shortened, and the cost is reduced greatly; a technical support is provided for the protection and large-scale industrialized production popularization and application of goodyera foliosa.

Description

technical field [0001] The invention belongs to a rapid propagation method of important plant materials in endangered wild plants, in particular to a tissue culture method of wild orchids. Background technique [0002] Goodyera foliosa (Lindley) Benth. belongs to the Orchidaceae Variegated Orchid, and is one of the species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora. It is a national second-class protected plant. Its distribution: China's Fujian, Guangdong, Guangxi, Sichuan, Taiwan, Tibet, and Yunnan, as well as Bhutan, India, Japan, the Korean Peninsula, Myanmar, Nepal, and Vietnam. In order to solve the problems in the prior art, such as weak natural reproduction ability, poor propagation and diffusion ability, difficulty in natural regeneration, and scarcity of high-quality seedlings. The invention adopts tissue culture technology to rapidly breed a large number of high-quality seedlings, which helps to solve the shortage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何碧珠林蔚刘江枫何官榕
Owner FUJIAN AGRI & FORESTRY UNIV
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