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Construction method of fingerprint of Ilex rotunda thunb medicinal material and detection method of Ilex rotunda thunb medicinal material

A technology of fingerprint spectrum and construction method, which can be used in measurement devices, material separation, analysis of materials, etc., and can solve the problems of large error and complex results.

Active Publication Date: 2015-07-08
GUANGZHOU XINGQUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bingying is dry bark. At present, there are many confusing products with similar appearance in Chinese medicinal materials. However, the method for detecting whether it is Bingying in the prior art is relatively complicated, and the error of the result is relatively large.

Method used

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  • Construction method of fingerprint of Ilex rotunda thunb medicinal material and detection method of Ilex rotunda thunb medicinal material
  • Construction method of fingerprint of Ilex rotunda thunb medicinal material and detection method of Ilex rotunda thunb medicinal material
  • Construction method of fingerprint of Ilex rotunda thunb medicinal material and detection method of Ilex rotunda thunb medicinal material

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Experimental program
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Effect test

Embodiment 1

[0048] Embodiment 1 The construction method of the fingerprint of the medicinal material Jiubing

[0049] 1. Instruments, reagents and materials

[0050] 1. Instrument

[0051] KQ-100B Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); T-214 Electronic Analytical Balance (Denver Company); Waterse2695 High Performance Liquid Chromatography System, Empower Workstation, including Quaternary Gradient Pump, Autosampler, UV Detection device (Waters Corporation).

[0052] 2. Reagents

[0053] Glacial acetic acid (chromatographically pure, Tianjin Institute of Chemical Reagents); methanol, acetonitrile (chromatographically pure, Honeywell Company); water is ultrapure water;

[0054] The reference substance was syringin reference substance (National Institute for the Control of Pharmaceutical and Biological Products, batch number 111574-200603).

[0055] 3. Materials

[0056] Jiubing medicinal materials (provided by Guangzhou Baiyunshan Xingqun (Pharmaceutical) Co., Ltd...

Embodiment 2

[0153] Example 2 The detection method of the fingerprint of the medicinal material Jiubing

[0154] The present embodiment a kind of detection method of the fingerprint chromatogram of the medicinal material that saves must, comprises the following steps:

[0155] (1) Preparation of reference substance solution:

[0156] Accurately weigh about 12 mg of the syringin reference substance, add an appropriate amount of methanol, and prepare a solution containing 1.22 mg of syringin per 1 ml of methanol as the reference solution.

[0157] (2) Preparation of the test solution:

[0158] Take about 1g of the medicinal material powder to be tested (batch number: 20140801, passed through a 60-mesh sieve), accurately weigh it, put it in a 100ml conical flask with a stopper, add 25ml of 50% ethanol precisely, seal it tightly, weigh it, and ultrasonically treat it (power 160W , frequency 59kHz) for 60 minutes, let cool, weigh again, make up the lost weight with 50% ethanol, shake well, fi...

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Abstract

The invention discloses a construction method of a fingerprint of Ilex rotunda thunb medicinal material and a detection method of the Ilex rotunda thunb medicinal material. The construction method comprises the following steps: (1) preparation of a reference solution; (2) preparation of a test solution; and (3) determination, namely respectively precisely sucking up the test solution and the reference solution, and injecting the sucked solutions into a high performance liquid chromatograph for determination, thereby obtaining the fingerprint of Ilex rotunda thunb medicinal material composed of 13 common characteristic peaks. For the construction method and the detection method, through a lot of experiments and research by the inventor, the method for preparing the test solution and the reference solution is determined, optimal parameters of chromatographic conditions of the high performance liquid chromatograph are also determined, and therefore, the fingerprint of the Ilex rotunda thunb medicinal material is constructed. With the fingerprint constructed by the method disclosed by the invention and the detection method, the Ilex rotunda thunb medicinal material can be identified accurately, precision is high, and a reagent is saved.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a construction method and a detection method of a fingerprint of a medicinal material of jiubing. Background technique [0002] Jiubiying is the dry bark of Ilex holly, which is bitter and cold in nature. Gas slightly fragrant, bitter, slightly astringent. It has the effects of clearing away heat and detoxification, promoting dampness and relieving pain. It is suitable for heat-damp fever, sore throat, damp-heat diarrhea, abdominal distension and pain, rheumatic arthralgia, eczema, boils, bruises, etc. Modern pharmacological studies have shown that jiubing also has anti-arrhythmia and anti-myocardial ischemia effects, anti-hypertensive and heart rate-lowering effects, anti-tumor effects, and hemostatic effects. [0003] Bingying is dry bark. At present, there are many confusing products with similar appearance in Chinese medicinal materials. However, the method f...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 蒋莉娟姚江雄许招懂方铁铮
Owner GUANGZHOU XINGQUN PHARMA
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