Histoplasma infection molecular diagnosis kit based on loop-mediated isothermal amplification (LAMP) technique principles and application thereof

Abstract

The invention relates to a histoplasma infection molecular diagnosis kit based on loop-mediated isothermal amplification (LAMP) technique principles and application thereof. By selecting appropriate target sequences, an appropriate LAMP primer set is obtained according to the ITS segment and M antigen gene. The primer set has the advantages of excellent specificity and high sensitivity, and thus, is suitable for early infection diagnosis of histoplasma and detection and identification of histoplasma in the sample. The primer set for diagnosing histoplasma is quick and simple, is high in operability and convenient for result interpretation, solves the problems of long time cycle for clinical histoplasma infection diagnosis, high requirements for detection personnel technology and laboratory platform conditions, and the like, can be prepared into a kit, and is suitable for wide popularization and application.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a molecular diagnosis kit for histoplasma infection based on the principle of loop-mediated isothermal amplification technology and its application. Background technique [0002] Histoplasma is a biphasic fungal genus commonly found in bird and bat feces, which in its natural state is a mycelial phase that transforms into a yeast phase in animals or humans. The genus Histoplasma comprises three species, including Histoplasma capsulatum, Histoplasma capsulatum var.duboisii, Histoplasma capsulatum var. farciminosum), in which H. equine capsularis causes lymphangitis epizootics in horses. Histoplasmosis (Histoplasmosis, HP) is caused by biphasic Histoplasma histoplasma, which is widely distributed around the world. African histoplasmosis caused by Histoplasma var. dunai and Histoplasma capsularis equine. [0003] Humans are often infected by inhaling fungal spores in ...

AI Technical Summary

Problems solved by technology

Its disadvantage is that the primer design requirements are relatively high, which is the focus of research breakthroughs

[0008] In summary, based on the sequence analysis of each DNA fragment of the HP strain genome, an appropriate target gene was screened out, and a technology for the early and rapid diagnosis of histoplasma infection based on the principle of LAMP technology was developed, which is useful for solving clinical problems of histoplasma infection. The long time period for the diagnosis of plasmabacterial infection and the high requirements for the testing personnel's skills and laboratory platform conditions are of great significance, which will provide convenience for early diagnosis and timely treatment in clinical practice

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