Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mimic enzyme, preparation method, application method and application of mimic enzyme

A technology for simulating enzymes and oxidases, which is applied in the field of nanotechnology and enzyme catalysis, which can solve the problems of cumbersome synthesis steps, low catalytic activity, and easy-to-use nanoparticles, and achieve excellent water solubility, high catalytic activity, and maintain catalytic activity. Effect

Active Publication Date: 2015-07-08
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF7 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of single performance of simulated enzymes in the prior art and easy aggregation of nanoparticles, and the reduction of active sites and low catalytic activity after coating the nanoparticles in order to prevent agglomeration, the synthetic steps of simulated enzymes are cumbersome and harsh conditions, etc., the present invention One of the purposes of the present invention is to provide a mimetic enzyme, which has the activities of oxidase, peroxidase and catalase at the same time, and is expected to replace related protein enzymes in the fields of immunoassay, biological detection and clinical diagnosis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mimic enzyme, preparation method, application method and application of mimic enzyme
  • Mimic enzyme, preparation method, application method and application of mimic enzyme
  • Mimic enzyme, preparation method, application method and application of mimic enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A mimetic enzyme based on a single-layer molybdenum disulfide-loaded platinum-copper alloy nanocomposite, obtained by the following method:

[0059] (1) Disperse the powdery molybdenum disulfide in the aqueous solution, and add 0.05wt% chitosan, and ultrasonically peel off to obtain the single-layer molybdenum disulfide aqueous solution modified by chitosan; the above-mentioned single-layer molybdenum disulfide, platinum chloride Disperse acid and copper acetate in 10mL of water to make the concentrations 2.4mg / L, 0.1mmol / L and 0.03mmol / L respectively, stir evenly, and place in an ice bath to cool for 20min to obtain molybdenum disulfide flakes, platinum Aqueous solutions of salts and copper salts;

[0060] (2) Add 1 mL of 9mmol / L sodium borohydride solution dropwise to the aqueous solution obtained in step (1) that is dispersed with molybdenum disulfide sheets, platinum salts and copper salts, the rate of addition is 60 μL / min, stir and continue to place Carry out red...

Embodiment 2

[0086] A mimetic enzyme based on a single-layer molybdenum disulfide-loaded platinum-copper alloy nanocomposite, obtained by the following method:

[0087] (1) Disperse powdered molybdenum disulfide in aqueous solution, and ultrasonically peel off to obtain a single-layer molybdenum disulfide aqueous solution; disperse single-layer molybdenum disulfide, chloroplatinic acid and copper chloride in 10mL water to make the concentrations 5mg respectively / L, 0.1mmol / L and 0.1mmol / L, stir evenly, place in an ice bath to cool for 20 minutes, and obtain an aqueous solution dispersed with molybdenum disulfide sheets, platinum salts and copper salts;

[0088] (2) Add 1mL of 12mmol / L sodium borohydride solution dropwise to the aqueous solution obtained in step (1) that is dispersed with molybdenum disulfide sheets, platinum salts and copper salts, the rate of addition is 30 μL / min, stir and continue to place Carry out reduction reaction in ice bath for 1h;

[0089] (3) After the reactio...

Embodiment 3

[0091] A mimetic enzyme based on a single-layer molybdenum disulfide-loaded platinum-copper alloy nanocomposite, obtained by the following method:

[0092] (1) Disperse powdered molybdenum disulfide in aqueous solution, and ultrasonically peel off to obtain a single-layer molybdenum disulfide aqueous solution; disperse single-layer molybdenum disulfide, chloroplatinic acid and copper acetate in 10mL water to make the concentrations respectively 4.5mg / L, 0.05mmol / L and 0.15mmol / L, stir evenly, place in an ice bath to cool for 15min, and obtain an aqueous solution dispersed with molybdenum disulfide sheets, platinum salts and copper salts;

[0093] (2) Add 10mmol / L sodium borohydride solution 1mL dropwise to the aqueous solution obtained in step (1) that is dispersed with molybdenum disulfide sheets, platinum salts and copper salts, the rate of addition is 50 μL / min, stir and continue to place Carry out reduction reaction in ice bath for 1h;

[0094] (3) After the reaction is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to mimic enzyme. The mimic enzyme is a molybdenum disulfide sheet layer of which the surface is loaded with platinum-copper alloy nanoparticles. The preparation method comprises the step of performing reduction reaction for a water solution in which the molybdenum disulfide sheet layer, platinum salt and copper salt to obtain the mimic enzyme that is the molybdenum disulfide sheet layer of which the surface is loaded with the platinum-copper alloy nanoparticles. The mimic enzyme has a plurality characteristics of activity of oxidase, peroxidase and catalase; different mimic enzyme activity can be used for creating different methods or applied to the unknown fields; the preparation method is simple and easy to be carried out; the conditions are mild; the one-step method can be carried out to reduce and prepare through a water-phase system.

Description

technical field [0001] The invention belongs to the field of nanotechnology and enzyme catalysis, and relates to a simulated enzyme, its preparation method, use method and application, and in particular to a kind of simulated enzyme activity (oxidase, peroxidase and catalase) which has three kinds of simulated enzyme activities simultaneously. The simulated enzyme, its preparation method, use method and application; the simulated enzyme is a composite material of single-layer molybdenum disulfide supported platinum-copper alloy nanostructure. Background technique [0002] Enzyme catalysis, as an important part of green chemistry, has become one of the most active research fields in the intersection of modern biology and chemistry. Mimetic enzymes are a kind of artificially synthesized catalysts with non-protein structure but similar catalytic activity to natural enzymes, which overcome the shortcomings of natural enzymes such as poor stability, variability and inactivation, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B22F9/24B01J27/051G01N21/78G01N21/31G01N21/25G01N33/53
Inventor 乞萃蔡双飞杨蓉王琛王新环
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com