Culture medium for producing laccase by fermenting lotus leaf lyophyllum liquid and method for producing laccase

Abstract

The invention provides a culture medium for producing laccase by fermenting lotus leaf lyophyllum liquid and a method for producing the laccase. The culture medium for producing the laccase mainly contains 12g/L to 28g/L of glucose, 1g/L to 2g/L of casein, 1g/L to 2g/L of a yeast extract, 2g/L to 3.5g/L of MgSO4, 0.0025g/L to 0.004g/L of CuSO4, 2.91g/L to 5.09g/L of KH2PO4, 0.1g/L of vitamin B and 0.012g/L of guaiacol, wherein the pH of the culture medium is 4.86 to 6.58, and the culture medium is prepared by using water. The culture medium for producing the laccase is sterilized for 30min at the temperature of 115 DEG C for later use. The culture medium for producing the laccase, disclosed by the invention, is used for producing the laccase by adopting lotus leaf lyophyllum; in comparison with the existing method for producing the laccase by using edible fungus, the culture medium is high in laccase activity, and the laccase activity is as high as 829830U/L. The method disclosed by the invention is simple in process, controllable in process, free of limitation of external environmental condition and applicable to large-scale industrial fermentation tank cultivation production. The fermentation level of the culture medium disclosed by the invention reaches or surpasses the fermentation level of other edible funguses.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a culture medium and a method for producing laccase by liquid fermentation of lotus leaves and mushrooms. Background technique [0002] Laccase (Laccase, E.C.1.10.3.2) was first discovered in the secretion of Lac sumac (Rhus vernicifera) by Japanese scholar Yoshida in 1883. In 1896, Bertrand also confirmed the existence of laccase in fungi, and for the first time Name it laccase. It represents the largest subgroup of blue multicopper oxidases (MCOs), which can directly utilize the redox ability of copper ions to catalyze the oxidation of various aromatic compounds accompanied by the reduction of oxygen molecules to water. The range of its catalytic substrates is very wide, so it is difficult to describe it by its reducing substrates. The typical substrates it can oxidize are hydroquinone and phenolic compounds. [0003] Laccase generally exists in the form of a single protein body. I...

AI Technical Summary

Problems solved by technology

The liquid medium used: 200 g of potato, 30 g of bran, 1 g of potassium dihydrogen phosphate, 3 g of peptone, 20 g of glucose, 1 L of water, natural pH, laccase-producing laccase medium, lotus leaf The enzyme production of laccase from the strain ZY 48-1 (also known as 1022) is 618.33U / mL, which is relatively low and wastes a lot of raw materials. It needs 200 grams of potatoes per liter as raw materials, and the potato culture medium It is troublesome in the production process, and potatoes need to be boiled, which is time-consuming and labor-intensive