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Anti-tumor angiogenesis immune composite peptide, and preparation method and application thereof

An angiogenesis and immune complex technology, applied in peptide preparation methods, biochemical equipment and methods, anti-tumor drugs, etc., to achieve effective immune response, high purity, and the effect of inhibiting tumor growth

Inactive Publication Date: 2015-07-01
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no reports of vaccines targeting the three targets of VEGF, PDGF and EPO at the same time. Therefore, it is necessary to provide a vaccine and therapeutic method that can simultaneously target the three targets of VEGF, PDGF and EPO.

Method used

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  • Anti-tumor angiogenesis immune composite peptide, and preparation method and application thereof
  • Anti-tumor angiogenesis immune composite peptide, and preparation method and application thereof
  • Anti-tumor angiogenesis immune composite peptide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] A preparation method of anti-tumor angiogenesis immune complex peptide, comprising the following steps:

[0035] 1. Polypeptide synthesis: a peptide synthesizer (CS536, CSBio, USA) was used to synthesize the amino acid sequence shown in SEQ ID NO: 7 to obtain the anti-tumor angiogenesis immune complex peptide.

[0036] 2. Instruments and conditions for peptide purification: Reverse high performance liquid chromatography (RP-HPLC) instrument Shimadzu high performance liquid chromatography system / Discovery C18 chromatographic column, setting detection wavelength: 220nm; purity 92%.

[0037] 3. Mass spectrometry to identify whether the molecular weight of the polypeptide is consistent with the theory: laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF MS) (BIFI EXTMIII Germany), the molecular weight is 12KD.

[0038] After the crude peptide was synthesized and purified by liquid chromatography, it was characterized by MALDI-TOF MS. The mass spectrometr...

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PUM

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Abstract

The invention provides an anti-tumor angiogenesis immune composite peptide. The anti-tumor angiogenesis immune composite peptide includes an immunogenicity epitope of a vascular endothelial growth factor (VEGF), an immunogenicity epitope of platelet-derived growth factor (PDGF) and an immunogenicity epitope of erythropoietin EPO. The anti-tumor angiogenesis immune composite peptide has the capability of targeting three target sites, VEGF, PDGF and EPO, thus effectively inhibiting tumor growth; in addition, the anti-tumor angiogenesis immune composite peptide can be used as an active immunization vaccine, and effectively reduce the antibody drug immunogenic response. The invention also provides a preparation method and application of the anti-tumor angiogenesis immune composite peptide.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to an anti-tumor angiogenesis immune complex peptide and its preparation method and application. Background technique [0002] Judah folkman put forward the theory that tumor growth depends on angiogenesis in 1971, thus creating a new era of tumor-dependent angiogenesis therapy. More and more evidence shows that tumor growth depends on angiogenesis, and vaccines targeting vascular-associated antigens can trigger the body to produce specific cellular or humoral immune responses, inhibit the formation of blood vessels, and thus prevent tumor cell growth and metastasis role. [0003] Vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF) and stimulating erythropoietin (EPO) are key factors in promoting tumor angiogenesis and are important regulators of inducing angiogenesis. In the process of tumor occurrence and development, the hypoxic environment of tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K1/06C12N15/12C12N15/63A61K38/18A61P35/00
Inventor 万晓春吕卫东
Owner SHENZHEN INST OF ADVANCED TECH
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