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Method for producing erythritol in fermentation tank

A technology of erythritol and fermentation tank, which is applied in the field of fermentation, can solve the problems of long operation time, time-consuming and labor-intensive infection probability, etc., and achieve the effect of reducing energy consumption, reducing the probability of infection, and saving manpower and time

Inactive Publication Date: 2015-06-24
ZHUCHENG DONGXIAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of primary seed inoculation, the seed bottle generally needs to combine 200 shake flasks, which takes a long time, is time-consuming and labor-intensive, and has a high chance of contamination

Method used

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  • Method for producing erythritol in fermentation tank
  • Method for producing erythritol in fermentation tank
  • Method for producing erythritol in fermentation tank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Inoculate erythritol-producing Candida lipolytica into 10 eggplant bottles (composition of slant medium: glucose 200g, yeast extract 10g, urea 1.0g, agar 20g, tap water 1000mL, pH6.0), 30℃ After culturing for 48 hours, add 100 mL of sterile water to each eggplant bottle, put all the washed bacterial lawn into a sterilized fermenter containing 20L medium for 18 hours, and then put all of them into the first-level seed tank and inoculate The amount is 1%, and after 20 hours, it is connected to the secondary seed tank, and the inoculation amount is 10%, and after 10 hours, it is connected to the fermenter, and the inoculation amount is 10%, and the erythritol content measured after 120 hours of fermentation is 160g / L.

Embodiment 2

[0024] Different from Example 1, in Example 2, Candida lipolytica producing erythritol was inoculated into a plurality of eggplant bottles, and the slant medium was composed of the following parts by weight: 150 parts of glucose, yeast 5 parts of ointment, 0.5 parts of urea, 15 parts of agar, 800 parts of tap water, adjust the pH value to 6.0, incubate at 28°C for 45 hours, add 100 mL of sterile water to each eggplant bottle, and put all the washed bacterial lawn into Cultivate in a sterilized fermenter containing 20L medium for 19 hours, all of which are connected to the first-level seed tank, and then pass through the second-level seed tank and fermenter to produce erythritol.

[0025]

Embodiment 3

[0027] Different from Example 1, in Example 2, Candida lipolytica producing erythritol was inoculated into a plurality of eggplant bottles, and the slant medium was composed of the following parts by weight: 250 parts of glucose, yeast 15 parts of ointment, 1.5 parts of urea, 25 parts of agar, 1200 parts of tap water, adjust the pH value to 6.0, incubate at 32°C for 50 hours, add 100 mL of sterile water to each eggplant bottle, and put all the washed bacterial lawn into Cultivate in a sterilized fermenter containing 20L medium for 18 hours, all of which are connected to the first-level seed tank, and then pass through the second-level seed tank and fermenter to produce erythritol.

[0028]

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Abstract

The invention relates to the field of microorganisms, and particularly relates to a method for producing erythritol by inoculating in a fermentation tank instead of seed flasks. The method disclosed by the invention comprises the following steps: inoculating candida lipolytica for producing erythritol to multiple eggplant flasks, wherein an inclined culture medium comprises the following components in parts by weight: 150-250 parts of glucose, 5-15 parts of a yeast extract, 0.5-1.5 parts of urea, 15-25 parts of agal-agal, and 800-1200 parts of tap water; after the pH value is adjusted to 6.0, culturing for 45-50 h at a temperature of 28-32 DEG C, and adding 80-120 parts of sterile water in each eggplant flask; and inoculating all lawns obtained by washing to a sterilized fermentation tank containing 20 L of the culture medium to culture for 15-22 h, completely inoculating the obtained objects to a primary seed flask, and then enabling the obtained products to pass through a secondary seed flask and the fermentation tank, so that erythritol is produced. The method disclosed by the invention is designed for producing erythritol by inoculating in the fermentation tank instead of seed flasks, thereby saving manpower and time, and greatly reducing the incidence of bacterial infection.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing erythritol in a fermenter. Background technique [0002] Erythritol is a natural zero-calorie sweetener, which has a refreshing feeling and unique taste when eaten. Since erythritol does not participate in sugar metabolism and blood sugar changes, it is suitable for diabetics. Erythritol does not cause dental caries, has a higher tolerance than xylitol, absorbs quickly, and has almost no possibility of causing gastrointestinal discomfort. Among edible sugar alcohols, erythritol is the only one produced by fermentation, which is closer to natural conversion and extraction. With the improvement of living standards, people pay more and more attention to food health care. Erythritol, as a health-care sugar with superior quality, its demand will increase all the year round. At present, most domestic fermentation st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/73
CPCC12P7/18
Inventor 王松江王荣峰
Owner ZHUCHENG DONGXIAO BIOTECH
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