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Liquefying inoculation method for edible and medicinal fungi using bean dregs as raw material

A technology for edible and medicinal fungi and bean dregs, which is applied in the directions of botanical equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of no residue and insufficient biomass in the medium, and achieves strong synchrony of mycelial growth and energy. The effect of reduced consumption and shorter incubation time

Inactive Publication Date: 2015-06-24
FUJIAN JIATIAN AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method of liquefaction and inoculation of edible and medicinal fungi using bean dregs as a raw material, which solves the problems of insufficient biomass of other solid mediums and the inability to achieve no residue in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A liquefaction inoculation method of Pleurotus ostreatus using bean dregs as raw material, which is specifically implemented according to the following steps: fresh bean dregs are dehydrated to 70%-80%, and used immediately or frozen. After removing water, add 1% calcium bicarbonate to the bean dregs, adjust the appropriate pH value according to the biological characteristics of different edible and medicinal fungi, and fully stir until it becomes a spongy medium with strong looseness. Inoculation of the mother species: spongy Put the culture medium into the test tube and shake gently to one-third, and use a flat iron bar of about 8 mm to press the culture material into an inclined plane, which is beneficial to connect the bacteria to the middle during inoculation, and obtain the fastest growth of mycelium Full; original seed inoculation: Use Erlenmeyer flasks, polypropylene (PP), polycarbonate (PC) plastic boxes, plastic bags and other containers with good transparency,...

Embodiment 2

[0020] A liquefaction inoculation method of Flammulina velutipes using bean dregs as raw materials, specifically implemented according to the following steps: fresh bean dregs are dehydrated to 70%-80%, and used immediately or frozen. After removing water, add 1% calcium bicarbonate to the bean dregs, adjust the appropriate pH value according to the biological characteristics of different edible and medicinal fungi, and fully stir until it becomes a spongy medium with strong looseness. Inoculation of the mother species: spongy Put the culture medium into the test tube and shake gently to one-third, and use a flat iron bar of about 8 mm to press the culture material into an inclined plane, which is beneficial to connect the bacteria to the middle during inoculation, and obtain the fastest growth of mycelium Full; original seed inoculation: Use Erlenmeyer flasks, polypropylene (PP), polycarbonate (PC) plastic boxes, plastic bags and other containers with good transparency, the be...

Embodiment 3

[0022] A Phellinus liquefaction inoculation method using bean dregs as raw materials, specifically implemented according to the following steps: fresh bean dregs are dehydrated to 70%-80%, and used immediately or frozen. After removing water, add 1% calcium bicarbonate to the bean dregs, adjust the appropriate pH value according to the biological characteristics of different edible and medicinal fungi, and fully stir until it becomes a spongy medium with strong looseness. Inoculation of the mother species: spongy Put the culture medium into the test tube and shake gently to one-third, and use a flat iron bar of about 8 mm to press the culture material into an inclined plane, which is beneficial to connect the bacteria to the middle during inoculation, and obtain the fastest growth of mycelium Full; original seed inoculation: Use Erlenmeyer flasks, polypropylene (PP), polycarbonate (PC) plastic boxes, plastic bags and other containers with good transparency, the best filling amo...

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Abstract

The invention discloses a liquefying inoculation method for edible and medicinal fungi using bean dregs as a raw material. The method includes the following steps of raw material handling, mother seed culture, stock culture, sterilization handling, inoculating, cultivating and liquefying. The liquefying inoculation method effectively solves the problems that the biomass of other solid media is insufficient and the residue-free purpose cannot be achieved, and has the advantages of being short in culture time, high in mycelial growth synchronism, large in number of loose gaps of the fungous material, and large in containing quantity of mycelium sheets. The cost and space are saved on the aspect of project investment, equipment is relatively simple, a culture room is saved by dozens of times, and the energy efficiency ratio is quite obvious. The investment is reduced by over 90% compared with liquid state fermentation type liquid strain equipment, the stability is high, and energy consumption is reduced by over 80%.

Description

technical field [0001] The invention belongs to the technical field of strain inoculation, and in particular relates to a liquefaction inoculation method of edible and medicinal fungi using bean dregs as raw materials. Background technique [0002] The current production of edible fungus strains mainly adopts three methods: traditional solid strains, Korean fermented liquid strains and solid liquefaction strains: traditional solid strains have high production costs such as raw materials, labor, and electricity costs, and the yield is low, which is difficult The problem of meeting the growing domestic demand for edible fungus production capacity; Korean traditional fermentation liquid strain fermenters, fermentation medium sterilizers, compressed air dehydration and aseptic treatment equipment, liquid inoculation machines and other equipment are expensive (current equipment In addition, the operation process requires high levels of personnel and space cleanliness, the technol...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 杨加金
Owner FUJIAN JIATIAN AGRI DEV
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