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Novel extraction process of coenzyme Q10

A new technology, coenzyme technology, applied in the direction of microorganisms, biochemical equipment and methods, organic chemistry, etc., can solve the problems of low bacterial concentration and product yield, reduced corporate profit margin, high production cost, etc., to achieve yield and purity Improvement, avoid pollution, low cost effect

Active Publication Date: 2015-06-10
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to current reports, when coenzyme Q10 is produced by microbial fermentation, the bacterial concentration and product yield are low, which makes the production cost high and the profit margin of the enterprise is greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of extracting coenzyme Q from fermented liquid 10 The novel process, it comprises the steps:

[0025] 1) Rhodotorula rubra ATCC74283 (see US5474919A), Rhodobacter sphaeroides ATCC17025 (see Journal of Biological Chemistry2001, V276, No44, P41161-41164) and Paracoccus denitrificans ATCC 19367 (see J.Bacteriol.April 1996V178, No7, p1866-1871) was cultured to a concentration of 1×108 / mL bacteria solution, mixed according to the volume ratio of 1:1:2 to obtain a mixed bacteria solution, and then According to 5% (v / v) inoculum size, inoculate in fermentation medium (glucose 10g, peptone 5g, (NH 4 ) 2 SO 4 5g, KH 2 PO 4 1.0g, Na 2 HPO 4 0.5g, MgSO 4 0.5g, water 1000mL, pH 7.0), culture temperature 29°C, 100rpm shaker culture for 30 hours to obtain fermentation broth, the measured dry cell weight was 120g / L;

[0026] 2) Centrifuge the fermentation broth at 4000rpm for 30min, separate the supernatant and the bacterial cells, wash the bacterial cells until neutr...

Embodiment 2

[0031] A kind of extracting coenzyme Q from fermented liquid 10 The novel process, it comprises the steps:

[0032] 1) Cultivate Rhodotorula rubra ATCC 74283, Rhodobacter sphaeroides ATCC 17025 and Paracoccus denitrificans ATCC 19367 to a concentration of 1×10 8 Each / mL of the bacterial solution was mixed according to the volume ratio of 1:1:2 to obtain the mixed bacterial solution, and then inoculated in the fermentation medium (glucose 10g, peptone 5g, (NH 4 ) 2 SO 4 5g, KH 2 PO 4 1.0g, Na 2 HPO 4 0.5g, MgSO 4 0.5g, water 1000mL, pH 7.0), culture temperature 29°C, 100rpm shaker culture for 30 hours to obtain fermentation broth, the measured dry cell weight was 120g / L;

[0033] 2) Centrifuge the fermentation broth at 5000rpm for 30min, separate the supernatant and the bacterial cells, wash the bacterial cells until neutral, dry them at 60°C and crush them; mix the crushed bacterial cells and n-hexane in a mass ratio of 2:2 Mix, mix and extract with magnetic stirring, a...

Embodiment 3

[0038] Choose 200 piglets of 60 days, be divided into two groups, every group of 100, wherein the experimental group is raised with the feed prepared in this embodiment 1, and the matched group is fed with Tongwei medium and large pigs; same. See Table 1 for the indicators detected after 9 weeks of feeding.

[0039] Table 1

[0040] indicators

[0041] Conclusion: The feed prepared by the invention has better feeding effect, daily gain and feed-to-weight ratio are better than common pig feed in the market, and the cost is lower than that of the commercial feed.

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PUM

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Abstract

The invention belongs to the technical field of biology and discloses a novel extraction process of coenzyme Q10. The novel extraction process of the coenzyme Q10 takes coenzyme Q10 fermentation liquid as a raw material and comprises the following steps: extracting through an organic solvent, carrying out base-alcohol saponification, carrying out chromatography through a silica gel column, crystallizing through absolute ethyl alcohol, and carrying out suction filtration and vacuum drying to prepare the coenzyme Q10 product. The process is simple to operate, low in pollution, low in requirements on instruments and equipment and low in cost.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to coenzyme Q 10 The extraction method, especially relates to a kind of extracting coenzyme Q from fermented liquid 10 new technology. Background technique [0002] coenzyme Q 10 It is a fat-soluble natural vitamin substance, which plays the role of delivering hydrogen in the living body, and is one of the indispensable important elements for living organisms. coenzyme Q 10 As an activator of cell metabolism and a natural antioxidant, it plays an important role in the treatment of cardiovascular diseases and can also improve human immunity. It has become a research hotspot in the field of biomedicine. In recent years, it is also widely used in health care products, cosmetics and food industries. [0003] Coenzyme Q 10 The production methods mainly include animal and plant tissue extraction, chemical synthesis and microbial fermentation. The animal and plant tissue extraction meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/66C07C50/28C07C46/10C12R1/645C12R1/01
Inventor 李学朋沈伟伟张健健安新义
Owner XINJIANG FUFENG BIOTECH
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