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Serum-free medium for stem cells

A serum-free medium, stem cell technology, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve unfavorable industrialization, scale, clinical application limitations of stem cells, controllability, poor reproducibility, etc. problem, to eliminate pathogen contamination, shorten the time, and increase the amplification speed.

Active Publication Date: 2015-06-10
JIANGSU PURECELL BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stem cell culture medium with serum is uncertain due to the composition of serum, and the controllability and reproducibility are relatively poor, which is not conducive to industrialization and scale; moreover, the potential pathogen contamination in animal serum makes the stem cell Clinical application is very limited

Method used

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  • Serum-free medium for stem cells
  • Serum-free medium for stem cells
  • Serum-free medium for stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the preparation of culture medium

[0056] Preparation of basal medium: Take a bag of DMEM dry powder, dissolve it in 800mL deionized water, and then add 3g of NaHCO 3 , adjust the pH of the medium to 7.2-7.4 with a pH meter, and set the volume to 1000 mL.

[0057] Medium additive of the present invention comprises following composition:

[0058]

[0059] Dissolve the above medium additives with the basal medium, then adjust the pH between 7.2-7.4 with a pH meter, filter and sterilize with a 0.22 μm filter, and store at 4°C in the dark.

Embodiment 2

[0060] Embodiment 2: the preparation of culture medium

[0061] Preparation of basal medium: Take a pack of DMEM / F12 dry powder, dissolve it in 800mL deionized water, and then add 3g of NaHCO 3 , adjust the pH of the medium to 7.2-7.4 with a pH meter, and set the volume to 1000 mL.

[0062] Medium additive of the present invention comprises following composition:

[0063]

[0064]

[0065] Dissolve the above medium additives with the basal medium, then adjust the pH between 7.2-7.4 with a pH meter, filter and sterilize with a 0.22 μm filter, and store at 4°C in the dark.

Embodiment 3

[0066] Embodiment 3: the preparation of culture medium

[0067] Preparation of basal medium: Take a pack of MEM dry powder, dissolve it in 800mL deionized water, and then add 3g of NaHCO 3 , adjust the pH of the medium to 7.2-7.4 with a pH meter, and set the volume to 1000 mL.

[0068] Medium additive of the present invention comprises following composition:

[0069]

[0070]

[0071] Dissolve the above medium additives with the basal medium, then adjust the pH between 7.2-7.4 with a pH meter, filter and sterilize with a 0.22 μm filter, and store at 4°C in the dark.

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Abstract

The invention relates to the biological field, in particular to a serum-free medium for stem cells. A medium additive comprises 5-30 g / L of human serum albumin, 1-10 g / L of plant-derived recombinant human serum transferring, 0.1-8 g / L of alpha1 acid glycoprotein, 0.1-8 g / L of alpha2-HS-glycoprotein, 0.05-5 g / L of apolipoprotein AI, 0.05-5 g / L of apolipoprotein AII, 0.01-2 g / L of hemopexin, 0.05-5 g / L of adenosine deaminase, 0.03-3 g / L of serine protease inhibitors, 0.01-3 g / L of transthyretin and 0.01-3 g / L of human insulin. According to the serum-free medium for stem cells, pathogen pollution from animal origins is eliminated, the components are defined, and therefore reproducibility and controllability of the serum-free medium are better than those of a serum medium; the effect of in-vitro culture of stem cells is superior to the effect of the serum medium, the expansion speed of stem cells is increased, and culture time for stem cells is greatly shortened.

Description

technical field [0001] The invention relates to the field of biology, in particular to a serum-free culture medium for stem cells. Background technique [0002] Stem cells have broad application prospects in regenerative medicine (cell therapy), drug screening and early embryonic development research, but their research still faces enormous challenges, the most critical of which is the culture reagents of stem cells. The development of stem cell culture medium has gone through a dynamic development process from standard feeder layer culture to conditioned medium culture, and finally to defined medium culture. Initially, stem cells were grown on trophoblast cells in a medium supplemented with fetal bovine serum (FBS) and animal-derived growth factors. However, these animal-derived ingredients can cause many potential dangers in clinical practice: (1) The immunogens and toxic proteins contained in them can stimulate immune responses and cause immune rejection. For example, ce...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/0789C12N5/077
Inventor 彭乐
Owner JIANGSU PURECELL BIOMEDICAL TECH CO LTD
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