Traditional Chinese medicine probiotics compound preparation for preventing and treating respiratory diseases of broilers
A technology of composite preparations and probiotics, which is applied in the direction of respiratory diseases, drug combinations, and medical preparations containing active ingredients, etc., can solve the problems of limited types, high prices, and slow curative effect of traditional Chinese medicine, and achieve slow onset, Effects of avoiding infection, promoting absorption and utilization
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Embodiment 1
[0039] Embodiment 1: the isolation of pathogenic bacteria
[0040] 1. Test material:
[0041] 1.1 Disease material: The larynx, trachea, air sac, and lungs of chickens suffering from respiratory diseases around Tai'an City were aseptically collected.
[0042] 1.2 Media: Normal Nutrient Broth Agar Medium (NA), Blood Agar Medium, Eosin-Maylan Agar Medium (EMB), Tryptone Soy Agar Medium (TSA), Tryptone Soy Broth Medium (TSB ), SS medium, MacConkey medium (MAC), and chocolate agar medium, all of which were purchased from Qingdao Haibo Company.
[0043] 0.005% nicotinamide adenine dinucleotide (NAD), purchased from Beijing Land Bridge Technology Co., Ltd.
[0044] 1.3 Biochemical trace identification tubes: lactose, glucose, arginine, xylose-gelatin, MR-VP, malonate, mannitol, dulcitol semi-solid, ONPG, Simon's citrate, urease, lysate Amino acid, sterile liquid paraffin, and Kovacs' indigo matrix kit were all purchased from Qingdao Haibo Company.
[0045] 1.4 Reagents for molec...
Embodiment 2
[0088] Embodiment 2: Drug susceptibility test of traditional Chinese medicine and probiotics
[0089] 1. Test material
[0090] 1.1 Single traditional Chinese medicine: Poria, Pinellia, Anemarrhena, Campanulaceae, Fangfeng, Andrographis paniculata, Black Plum, Forsythia, Pomegranate Peel, Scutellaria, Scutellaria, Qinpi, Daqingye, Huanglian, Magnolia officinalis, Banlangen, Honeysuckle, Chai Hu, licorice, Houttuynia cordata, ephedra. The above-mentioned traditional Chinese medicines were all purchased from Wanning Pharmacy in Taishan District, Tai'an.
[0091] 1.2 Probiotics:
[0092] Bacillus: Bacillus subtilis JYM 841, Bacillus subtilis B7348.
[0093] Lactic acid bacteria: Pediococcus pentosaceae PP, Enterococcus faecalis SRG, Lactobacillus plantarum LP.
[0094] Bacillus subtilis (Bacillus subtilis) JYM841 has been preserved in China Center for Type Culture Collection on January 10, 2014, and the preservation number is CCTCC NO: M 2014012; Bacillus subtilis B7348 (Baci...
Embodiment 3
[0132] Embodiment 3: Screening of Chinese medicine probiotic compound preparation
[0133] 1. Test material
[0134] 1.1 Experimental animals: 80 1-day-old healthy broiler chickens were purchased from Taian Daewoo Breeding Plant.
[0135]1.2 Experimental diet: purchased from Taian Liuhe Feed Factory, the feed type is 510, 511. 1-21 days old were fed 510, 22-42 days old were fed 511.
[0136] 1.3 Test drugs:
[0137] Chinese medicine compound prescription F1, F2 prepared in embodiment 2;
[0138] Probiotics: Lactobacillus plantarum LP bacteria powder, Bacillus subtilis B7348 bacteria powder. The bacterial powder was provided by Shandong Baolai Lilai Biological Engineering Co., Ltd.
[0139] 1.4 Test reagents:
[0140] Serum biochemical index determination reagents: serum factor total protein (TP), albumin (ALB), urea nitrogen (BUN) kits, purchased from Kehua Biological Company.
[0141] 10% (v / v) neutral formalin fixative: anhydrous disodium hydrogen phosphate 6.5g / L, sod...
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