A kind of beer compound enzyme containing fungal α-amylase and preparation method thereof
A beer composite enzyme and amylase technology, which is applied in the directions of biochemical equipment and methods, enzymes, lyases, etc., can solve the problems of poor thermal stability of fungal α-amylase, low antioxidant capacity of composite enzymes, and incomplete enzyme systems. To achieve the effect of ensuring food safety and improving the utilization rate of raw materials
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Embodiment 1
[0078] Embodiment 1 raw material preparation
[0079] 1. Preparation of plant extracts
[0080] The preparation method of the plant extract is as follows: uniformly mix barley malt, wheat malt, and caramel malt according to the mass ratio of 4:2.5:1.5, and grind them to a particle size of 0.8mm to obtain crushed malt; then put papaya, pineapple, and figs respectively In an ultrasonic cleaning machine equipped with 0.6% sodium bicarbonate solution, perform ultrasonic cleaning for 8 minutes under the conditions of power 200W and frequency 30KHz, drain, crush to a particle size of 0.8mm at room temperature, and mix evenly according to the mass ratio of 4:4:2, add The raw material mixture is obtained by crushing malt with 2.2 times the mass of the mixture, adding water twice as much as the raw material mixture, adjusting the pH value to 3.5 with citric acid, and carrying out microwave extraction under the conditions of power 200W and frequency 2000Hz, wherein the total time of eac...
Embodiment 2
[0125] Embodiment 2 A kind of beer complex enzyme containing fungal α-amylase, is made up of the raw material of following parts by weight:
[0126] 50 parts of concentrated malt enzyme, 35 parts of amylase, 25 parts of protease, 22 parts of hemicellulase, 17 parts of plant extract, 16 parts of concentrated wort powder, 8 parts of esterase, 4 parts of protective agent, 4 parts of activator, anti 2 parts of oxidizing agent;
[0127] The amylase is uniformly mixed with an enzyme preparation composed of the following mass percentages: 40% glucoamylase, 30% fungal α-amylase, 15% pullulanase, 10% β-amylase, 5% medium temperature α-amylase Amylase;
[0128] The protease is uniformly mixed with an enzyme preparation composed of the following mass percentages: 55% neutral protease, 35% acid protease, 10% proline protease;
[0129] The hemicellulase is formed by uniform mixing of enzyme preparations composed of the following mass percentages: 45% heat-resistant β-glucan complex enzym...
Embodiment 3
[0137] Embodiment 3 A kind of beer complex enzyme containing fungal α-amylase, is made up of the raw material of following parts by weight:
[0138] 48 parts of concentrated malt enzyme, 32 parts of amylase, 20 parts of protease, 15 parts of hemicellulase, 16 parts of plant extract, 15 parts of concentrated wort powder, 5 parts of esterase, 3 parts of protective agent, 3 parts of activator, antioxidant 1.5 copies;
[0139] The activator is formed by uniformly mixing inorganic salts with the following mass components: 35 parts of zinc chloride, 15 parts of calcium chloride, 10 parts of sodium sulfate, and 5 parts of magnesium chloride;
[0140] Described protecting agent is made up of the raw material of following parts by weight: trehalose 30 parts, ganoderma polysaccharide 25 parts, NaCl 10 parts, (NH 4 ) 2 SO 4 8 parts, 8 parts of cysteine;
[0141] The antioxidant is that grape seed proanthocyanidins, rosemary extract and apricot leaf extract are uniformly mixed in a m...
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