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Scutellaria barbata extract as well as preparation method and application thereof

A technology of Scutellaria barbata extract and medicine, which is applied in the direction of medical formula, plant raw materials, medical preparations containing active ingredients, etc., and can solve the anti-EBV virus cleavage of the chloroquine-type diterpene component of Scutellaria barbata. Replication activity and other issues, to achieve a good inhibitory effect, the effect of great application prospects

Inactive Publication Date: 2015-05-27
广州市爱菩新医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the anti-EBV virus cleavage and replication activity of Scutellaria barbata extract and its neocrotane diterpene components in the prior art

Method used

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  • Scutellaria barbata extract as well as preparation method and application thereof
  • Scutellaria barbata extract as well as preparation method and application thereof
  • Scutellaria barbata extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The method for preparing compound 1-7 from barbata (Scutellaria barbata):

[0038] Scutellaria barbata (Scutellaria barbata) whole plant (3.5Kg), dried in the sun, pulverized, leached 3 times with 95% ethanol at room temperature, each time for 1 day, combined the concentrated solutions to obtain crude extract (362g), respectively washed with petroleum ether , ethyl acetate, n-butanol extraction, ethyl acetate layer (183g) directly through macroporous resin (D101), with ethanol / water (0:100, 10:90, 20:80, 30:70, 40:60 , 50:50, 60:40, 70:30, 80:20:, 95:5, 100:0, V / V) gradient elution, one part was collected for each 450mL, and the same part was combined for TLC detection to obtain a total of six Fraction Fr.1-6. Parts Fr.3-5 were separated repeatedly through silica gel column chromatography (200-300 mesh), SephadexLH-20, C18 column and semi-preparative liquid chromatography column to obtain the following 7 compounds.

[0039] Compound 1 (new compound):

[0040]

[0...

Embodiment 3

[0074] Example 3 Compound 1-7 of the present invention is tested for host cell toxicity

[0075] (1) Cell culture: P3HR-1 cells (primary exudative lymphoma cell line, containing EBV in latent infection stage) were cultured in vitro. The RPMI1640 medium containing 10% fetal bovine serum, 400ug / ml G418, and 100ng / ml doxycycline was used for routine maintenance and passage at 37°C and 5% carbon dioxide concentration.

[0076] (2) Test method: adjust the density of P3HR-1 cells in the logarithmic growth phase to 3×10 5 cells / ml, cells were treated with different concentrations of compound 1-7 (150, 100, 50, 20, 10, 1, 0.1, 0 μM), and three parallel wells were set up for each concentration. After 2 days, trypan blue was used to stain the cells, and under a light microscope Count the number of viable cells.

[0077] (3) Result processing: according to the formula: relative toxicity = 1-live cell compound+ / live cell compound_ Calculate the relative toxicity and median lethal dos...

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Abstract

The invention belongs to the technical field of Chinese herbal medicine extraction and in particular discloses a scutellaria barbata extract as well as a preparation method and application thereof. Three compounds in a new structure and four compounds in a known structure are separated from the scutellaria barbata extract. The seven compounds have good inhibiting effects on EB (Epstein-Barr) virus replication and have low toxic and side effects; therefore the seven compounds have great application prospects in preparing medicines for preventing EB virus infection.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicinal materials, and more specifically relates to an extract of Scutellaria barbata and its preparation method and application. Background technique [0002] Human herpesviruses can be divided into α, β and γ subtypes according to their biological characteristics, among which Epstein-Barr virus (Epstein-Barr virus) belongs to γ ​​subtype, also known as type 4 human herpesvirus (HHV-4). Epstein-Barr virus was first discovered by Epstein and Barr in 1964 from a lymphoma cell line cultured in vitro from African children's malignant lymphoma (Burkitt lymphoma), and a new herpes virus was discovered by electron microscopy, and it was named Epstein-Barr virus. The genome of EBV is a linear double-stranded DNA with a length of 172kb and encodes about 100 genes. Like human herpesviruses, EBV has two life cycles, a latent and lytic phase. In the latent phase, EBV expresses o...

Claims

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Application Information

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IPC IPC(8): A61K36/539A61P31/22C07D307/58A61K31/365
Inventor 顾琼吴泰宗徐峻袁岩王倩王彦
Owner 广州市爱菩新医药科技有限公司
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