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Human cytomegalovirus active infection rapid detection method and reagent kit for detection method

A technology of human cytomegalovirus and detection method, which is applied in the field of rapid detection method of active infection of human cytomegalovirus and the kit used for the detection method, and can solve the problems of lack of cellular immunity and the like

Active Publication Date: 2015-05-20
BIOHIT BIOTECH HEFEI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Simultaneous detection of pp65 antigenemia can not only indicate the presence of HCMV reactivation infection, but also the lack of cellular immunity against HCMV in patients

Method used

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  • Human cytomegalovirus active infection rapid detection method and reagent kit for detection method
  • Human cytomegalovirus active infection rapid detection method and reagent kit for detection method
  • Human cytomegalovirus active infection rapid detection method and reagent kit for detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] Assembly and use of HCMV pp65 antigenemia detection kit

[0038] 1. Assembly of kit

[0039] 1. Preparation of control slides

[0040] Collect an appropriate amount of peripheral blood from healthy people, separate lymphocytes with lymphocyte separation medium, and count the cells for later use; collect HEK293 cells stably expressing the HCMV pp65 gene, perform cell counting, and mix them with normal human lymphocytes at a ratio of 1:10000 as positive As a control, normal human lymphocytes were used as a negative control. Coat 2×10 on the position marked “+” on the slide 5 Positive control cells, spread 2×10 at the position marked “-” 5 positive and negative control cells. After drying at room temperature, fix with fixative at room temperature for 15 min, freeze-dry and seal.

[0041] 2. Preparation of anti-HCMV pp65 monoclonal antibody application solution

[0042] After purifying the monoclonal antibodies produced by the two hybridomas H0105 and F0249, prepare a...

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Abstract

The invention discloses a human cytomegalovirus active infection rapid detection method and a reagent kit for the detection method. The human cytomegalovirus active infection rapid detection method and the reagent kit are mainly used for rapidly monitoring and diagnosing of human cytomegalovirus active infection of pregnant women, newborn and people with immunocom promise including transplantation patients, HIV suffers, tumor patients, diabetics patients and the old people with the immune function decreased due to the age factor. The invention also relates to the preparation of a reagent kit utilizing the detection method.

Description

technical field [0001] The invention belongs to the field of antibody engineering and diagnostic methods, and in particular relates to a rapid detection method for active infection of human cytomegalovirus and a kit for the detection method. Background technique [0002] Human cytomegalovirus (human cytomegalovirus, HCMV) infection is very common in the population, and it is distributed worldwide. About 90% of adults in China can detect HCMV antibodies. Once HCMV is infected, it will remain latent in the host body for life. In the population with normal immune function, the virus and the host are in a "balanced" state. When the body is immunocompromised due to various reasons (HIV infection, transplantation and aging, etc.), this " "Balance" will be broken to cause HCMV reactivation infection, and the virus causing reactivation infection can come from the host itself or from other infected persons. Primary infection and reactivated infection are collectively referred to as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569C07K16/08
CPCG01N33/569G01N2333/045
Inventor 王明丽甘霖刘峰
Owner BIOHIT BIOTECH HEFEI
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