ZmLAX3 protein, and coding gene and application thereof

A protein and coding technology, applied in the field of ZmLAX3 protein and its coding gene and application, can solve the problems of less QTL and increased versatility of molecular marker-assisted selection

Active Publication Date: 2015-05-20
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although several QTL loci have been mapped on maize yield and yield composition traits (Shi Yunsu, Genetic diversity analysis of important maize inbred lines and QTL research on yield-related traits, 2008; Toledo et al., Genet.Mol.Res., 2011 , 10, 2133-2139; Lu et al., J Integr. Plant Biol., 2006, 48, 1233-1243; Veldboom et al., Theor Appl Genet, 1994, 89, 451-458), but these QTLs are rarely in Co-detected in different germplasm (temperate germplasm and tropical germplasm), and there is a significant interaction with the environment, there are few stable QTLs between environments, which increases the challenge of the versatility of molecular marker-assisted selection (Luna et al., Mol Breed, 2006, 17, 227-239)

Method used

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  • ZmLAX3 protein, and coding gene and application thereof
  • ZmLAX3 protein, and coding gene and application thereof
  • ZmLAX3 protein, and coding gene and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Embodiment 1, ZmLAX3 gene cloning

[0079] 1. Extraction and purification of corn RNA

[0080] Utilize the Trizol method to extract the total RNA of corn inbred line Qi 319 (hereinafter also referred to as wild-type corn), and the specific method steps are as follows:

[0081] Take a DEPC-treated 1.5ml centrifuge tube, add 1ml Trizol; take 100mg of corn material, place it in liquid nitrogen and grind it repeatedly to powder, transfer it to the above centrifuge tube, mix well, and let stand at room temperature for 5 minutes; 12000rpm, 4°C , centrifuge for 10 minutes, transfer the supernatant to a new centrifuge tube, add 200 μl chloroform, shake vigorously for 15 seconds, let stand at room temperature for 3 minutes; centrifuge at 12000 rpm, 4°C for 15 minutes, take the supernatant to a new centrifuge tube ;Add 250μl isopropanol and 250μl high salt solution, mix by inverting, and let stand at room temperature for 10 minutes; 12000rpm, 4℃, centrifuge for 10 minutes, pour ...

Embodiment 2

[0093] Example 2, Functional Research of ZmLAX3 Gene

[0094] 1. Construction of plant recombinant vector pZP211 UBI::ZmLAX3

[0095] The RNA of maize inbred line Qi 319 was extracted, reverse-transcribed to obtain cDNA as a template, and PCR was amplified with upstream primers and downstream primers to obtain a 1897bp PCR amplification product of ZmLAX3 gene.

[0096] Restriction endonucleases BamHI and Sac Ⅰ double-digest the ZmLAX3 gene PCR amplification product, and BamHI and Sac Ⅰ double-digest the pZP211::UBI expression vector "Cloning of ZmAATP Gene and Construction of Genetic Transformation Vector in Maize", Shandong Journal of Agricultural University (Natural Science Edition), 2012, 43(3) 321-327; the public can obtain it from Shandong Agricultural University), and the operation steps are carried out according to the instructions of T4 DNA ligase of Fermentas company. Then the ligation product was transformed into Escherichia coli DH5α competent cells, and cultured o...

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PUM

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Abstract

The invention discloses a ZmLAX3 protein, and a coding gene and application thereof. The invention provides a protein disclosed as 1) or 2): protein disclosed as Sequence 2 in the sequence table; and 2) Sequence-2-derived protein subjected to substitution and / or deletion and / or addition of one or more nucleotide residues with the same functions as the nucleotide sequence disclosed as Sequence 2 in the sequence table. The experiment proves that the cloning of the ZmLAX3 gene detects that the transgenic crop obtained from the gene has plant types, fringe character change and other phenotypes. The transgenic positive plant can improve the plant types, fringe characters and other important agronomic characters when being used for production, is used for enhancing the yield and quality of the crops, and has important economic value and social benefits.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a ZmLAX3 protein and its coding gene and application. Background technique [0002] With the rapid growth of the world economy, corn, as an important crop with multiple uses such as grain, feed, and industrial processing, has seen a sharp increase in global demand. China is a major corn producing country in the world, and increasing corn production has become an urgent problem to be solved in my country's corn industry, and it is also an urgent need for agricultural production and social economy. [0003] Yield trait is one of the important agronomic traits of maize. It is a quantitative trait controlled by multiple genes. There are complex interactions between genes. Gene expression is easily affected by the environment. It shows continuous variation of traits, and the genetic basis is very complex. Maize yield traits are the result of the combined effects of ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 赵翔宇张宪省蒋瑞捷别晓敏刘娜
Owner SHANDONG AGRICULTURAL UNIVERSITY
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