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Compositions and methods related to prevention and treatment of rabies infection

A technology for rabies and rabies virus, applied in the field of preparation of anti-rabies antibodies, can solve the problems of interfering with vaccine antigenicity, reducing efficacy and the like

Active Publication Date: 2015-05-06
万机创科(香港)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is advantageous that the antibodies administered in conjunction with the rabies vaccine do not interfere with the antigenicity of the vaccine, thereby reducing its efficacy

Method used

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  • Compositions and methods related to prevention and treatment of rabies infection
  • Compositions and methods related to prevention and treatment of rabies infection
  • Compositions and methods related to prevention and treatment of rabies infection

Examples

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example

[0161] In order to more fully illustrate selected embodiments of the invention, the following examples are presented. These examples should in no way be construed as limiting the scope of the invention as defined by the following claims.

example 1

[0163] Example 1 - Preparation and Characterization of Murine Rabies Virus Neutralizing Antibodies

[0164] Murine rabies virus neutralizing antibodies can be obtained by culturing hybridomas which in turn can be obtained by immunizing mice with rabies glycoprotein and subsequently fusing spleen cells or lymph node cells from the mice containing mouse myeloma cells. Referring to the above steps, the procedure for preparing anti-rabies antibodies is described in detail below. This method of producing the antibody of the present invention is merely illustrative of the production method and is not limited thereto. Other known procedures can be followed.

[0165] The present invention utilizes rabies virus glycoprotein (Genbank Accession No. ABY1950) as an immunogen for inducing antibodies capable of neutralizing rabies virus. The prepared immunogen is mixed with an adjuvant (eg, Freund's complete or incomplete adjuvant) and administered to mice. Suitable routes of administrati...

example 2

[0171] Binding activity of example 2-murine rabies virus neutralizing antibody

[0172] In this example, the binding activity of five rabies virus neutralizing antibodies (RVNA) to the rabies virus RV glycoprotein was investigated. The murine RVNA and other biological materials used in Examples 1-6 are shown in Table 3. Animals used in these studies included BALB / c mice, female, 6-8 weeks, weighing 20 to 30 grams, SPF; and Syrian hamsters, 2-3 months, weighing 100 grams, SPF.

[0173]

[0174] Binding curves of five RVNAs to RV glycoproteins as determined by indirect chemiluminescence enzyme immunoassay (CLEIA) are shown in figure 1 middle. Glycoproteins were diluted 1:500 in PBS and then coated with microplates. The five clones of RVNA were diluted to 10000, 2000, 400, 80, 16, 3.2 and 0.64 ng / mL, respectively. Goat anti-mouse IgG2a-FIRP and goat anti-mouse IgG2b-FIRP were used as enzyme-conjugated secondary antibodies. Relative luminescence units (RLU) represent the c...

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Abstract

The present disclosure relates generally to anti-rabies antibodies that can bind to and neutralize rabies virus. Antibodies of the present technology are useful alone or in combination with therapies known in the art for the treatment or prevention of rabies infection.

Description

technical field [0001] The present technology generally relates to the preparation of anti-rabies antibodies and their use. Specifically, the present invention relates to the preparation of rabies virus neutralizing antibody and its use in the prevention and treatment of rabies infection. Background technique [0002] The following descriptions are provided to aid the reader's understanding. Neither the information presented nor the references cited is an admission that it is prior art with the methods of the present invention. [0003] Rabies is an almost worldwide viral infection that primarily affects wild and domestic animals, but also humans. The infection causes a devastating and (if untreated) almost always fatal encephalitis. Over 70,000 people die from rabies infection each year, and millions more require post-exposure treatment. [0004] Rabies virus is an enveloped single-stranded RNA virus of the family Rhabdoviridae and the genus Lyssavirus. The genome of r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13A61K39/42A61P31/14
CPCA61K2039/505C07K2317/24C07K2317/76A61P31/14C07K16/10A61K39/395C07K2317/21G01N33/56983G01N2333/145G01N2469/10
Inventor 沈恩允任世奇
Owner 万机创科(香港)有限公司
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