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Preparation method and application of immunosensor established based on manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube

A technology of multi-walled carbon nanotubes and silver nanoparticles, which is applied in the direction of instruments, scientific instruments, and material inspection products, can solve the problems of cumbersome detection methods, complicated operations, and high detection limits, so as to reduce the detection limit, improve sensitivity, Effect of low detection limit

Inactive Publication Date: 2015-04-29
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many detection methods for tumor markers, such as radioimmunoassay, immunoradiometric assay, enzyme-labeled immunoassay, chemiluminescent assay, time-resolved fluorescent immunoassay, etc., but most of the detection methods are cumbersome, complicated to operate, and expensive Expensive, high detection limit, therefore, it is of great significance to establish a fast, simple and sensitive detection method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 A preparation of an immunosensor based on manganese dioxide-loaded silver nanoparticles multi-walled carbon nanotubes, the steps are as follows:

[0037] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0038] (2) Drop-coat 6 μL, 1.0 mg / mL β-cyclodextrin / multi-walled carbon nanotubes on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0039] (3) Continue to add 6 μL, 5 μg / mL primary tumor marker antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4°C;

[0040](4) Continue to add 3 μL of bovine serum albumin BSA solution with a mass fraction of 0.5% to the surface of the electrode dropwise to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C; Add 6 μL, 1pg / mL...

Embodiment 2

[0042] Example 2 A preparation of an immunosensor based on manganese dioxide-loaded silver nanoparticles multi-walled carbon nanotubes, the steps are as follows:

[0043] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0044] (2) Drop-coat 6 μL, 2.0 mg / mL β-cyclodextrin / multi-walled carbon nanotubes on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0045] (3) Continue to add 6 μL, 8 μg / mL primary tumor marker antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4°C;

[0046] (4) Continue to add 3 μL of bovine serum albumin BSA solution with a mass fraction of 1.0% dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse with ultrapure water, and dry in a refrigerator at 4°C; Add 6 μL, 1pg / mL~10 ng / mL of...

Embodiment 3

[0048] Example 3 A preparation of an immunosensor based on manganese dioxide-loaded silver nanoparticles multi-walled carbon nanotubes, the steps are as follows:

[0049] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0050] (2) Drop-coat 6 μL, 3.0 mg / mL β-cyclodextrin / multi-walled carbon nanotubes on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0051] (3) Continue to add 6 μL, 12 μg / mL primary tumor marker antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4°C;

[0052] (4) Continue to add 3 μL of bovine serum albumin BSA solution with a mass fraction of 2.0% to the surface of the electrode dropwise to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C; Add 6 μL, 1pg...

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Abstract

The invention belongs to the technical field of nano functional materials, immunoassay and biosensing, and provides a preparation method and application of an immunosensor established based on a manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube. The electrochemical immunosensor which is prepared by taking the manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube AgNPS@MnO2@MWCNTs as a detection antibody marker has the advantages of high specificity, high sensitivity, low detection limit and the like, and has significant scientific meanings and application values in detection on carcino-embryonic antigen CEA and alpha fetoprotein AFP.

Description

technical field [0001] The invention belongs to the technical fields of nanometer functional materials, immune analysis and biosensing, and provides a preparation method and application of an immunosensor based on manganese dioxide-loaded silver nanoparticle multi-walled carbon nanotubes. Background technique [0002] Tumors have a high incidence rate, rapid growth and metastasis, and are extremely harmful to human health. Tumor markers are metabolites produced and released by tumor cells in the form of antigens, enzymes, hormones, etc. that exist in tumor cells or in the body fluid of the host. It has a great impact on the differential diagnosis of malignant tumors, the judgment of the degree of tumor development, the observation and evaluation of tumor treatment effects, and the prediction of tumor recurrence and prognosis, and has attracted widespread attention. [0003] Common tumor markers such as AFP and CEA can play a certain role in the diagnosis of liver cancer. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/574G01N33/68
Inventor 李月云韩健姜丽萍李法瀛刘青刘会董云会
Owner SHANDONG UNIV OF TECH
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